Title
Changes in the sequence content of albumin mRNA and in its translational activity in the rat liver with age
Author
Horbach, G.J.M.J.
Princen, H.M.G.
van der Kroef, M.
van Bezooijen, C.F.A.
Yap, S.H.
Publication year
1984
Abstract
To investigate the regulation of age-related changes in albumin synthesis in the rat liver, total postnuclear RNA and polyribosomes, both membrane-bound and free, were prepared from livers of rats of different ages. By the use of a specific complementary DNA probe, the albumin mRNA sequence content was quantitated in these RNA fractions. These studies showed a specific increase in albumin mRNA sequence content in total postnuclear RNA and membrane-bound polyribosomes at between 12 and 24 months of age. Between 24 and 36 months of age, the increase in the amount of albumin mRNA in these two fractions was due only to an increase in liver weight. The increase in albumin mRNA sequence content was not found in the poly(A)+ fraction but in the RNA extracted from the void of oligo(dT)-cellulose column chromatography. The isolated polyribosomes were translated in a cell-free system to assess age-related changes in total protein and albumin synthesis due to translational control. No changes with age were found in the translational capacity of membrane-bound and free polyribosomes per RNA unit. Immunoprecipitation of the synthesized albumin in the translation products revealed that albumin synthesis in the cell-free system is not increased proportionally with the elevated albumin mRNA level between 12 and 24 months of age. This indicates that albumin mRNAs present in the livers of old rats are biologically less active than those found in younger animals. Chemicals/CAS: DNA, 9007-49-2; RNA, 63231-63-0; Amino Acids; DNA, 9007-49-2; RNA, Messenger; Serum Albumin
Subject
Rna
Albumin messenger ribonucleic acid
Animal cell
Nonhuman
Aging
Amino Acids
Animal
Base Sequence
Centrifugation, Density Gradient
DNA
Female
Liver
Nucleic Acid Hybridization
Polyribosomes
Rats
RNA, Messenger
Serum Albumin
Support, Non-U.S. Gov't
Translation, Genetic
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DOI
https://doi.org/10.1016/0167-4781(84)90078-2
TNO identifier
229586
ISSN
0167-4781
Source
Biochimica et Biophysica Acta - Gene Structure and Expression, 783 (1), 60-66
Document type
article