Title
Autoantibodies against alpha B-crystallin, a candidate autoantigen in multiple sclerosis, are part of a normal human immune repertoire
Author
van Noort, J.M.
Verbeek, R.
Meilof, J.F.
Polman, C.H.
Amor, S.
TNO Kwaliteit van Leven
Publication year
2006
Abstract
Human T-cell responses to the stress protein alpha B-crystallin in multiple sclerosis (MS)-affected brain samples are dominant when compared to other myelin antigens. The establishment of the apparent autoimmune repertoire against this antigen has been suggested to involve cross-priming during viral infection. Yet, another possibility would be that determinant spreading during ocular inflammation could generate a response to alpha B-crystallin, since it is also a major component of the eye. In this study, we compared serum IgG, IgA and IgM repertoires against a range of eye lens-derived ocular antigens using sera from healthy control subjects and MS patients with or without uveitis. This comparison revealed that among ocular antigens, alpha B-crystallin is the dominant target antigen for serum autoantibodies in both MS patients and healthy controls. Uveitis generally did not affect the antibody reactivity profile. These data provide further support for the notion that a normal adult human immune system is selectively reactive to alpha B-crystallin and they indicate that this responsiveness is unlikely to result from determinant spreading following ocular inflammation. © 2006 Edward Arnold (Publishers) Ltd. Chemicals / CAS: immunoglobulin G, 97794-27-9; immunoglobulin M, 9007-85-6; alpha-Crystallin B Chain; Autoantibodies; Autoantigens; Eye Proteins
Subject
Health
Biomedical Research
Alpha B-crystallin
Determinant spreading
Alpha crystallin
Autoantibody
Immunoglobulin A
Immunoglobulin G
Immunoglobulin M
Antigen antibody reaction
Cellular immunity
Clinical article
Controlled study
Disease association
Eye inflammation
Immune response
Immunoglobulin blood level
Immunoreactivity
T lymphocyte
Adult
alpha-Crystallin B Chain
Antibody Specificity
Autoantibodies
Autoantigens
Chromatography, High Pressure Liquid
Electrophoresis, Polyacrylamide Gel
Eye Proteins
Female
Humans
Immune System
Lens, Crystalline
Male
Middle Aged
Multiple Sclerosis
Uveitis
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DOI
https://doi.org/10.1191/135248506ms1271oa
TNO identifier
239300
ISSN
1352-4585
Source
Multiple Sclerosis, 12 (3), 287-293
Document type
article