Print Email Facebook Twitter Autoantibodies against alpha B-crystallin, a candidate autoantigen in multiple sclerosis, are part of a normal human immune repertoire Title Autoantibodies against alpha B-crystallin, a candidate autoantigen in multiple sclerosis, are part of a normal human immune repertoire Author van Noort, J.M. Verbeek, R. Meilof, J.F. Polman, C.H. Amor, S. TNO Kwaliteit van Leven Publication year 2006 Abstract Human T-cell responses to the stress protein alpha B-crystallin in multiple sclerosis (MS)-affected brain samples are dominant when compared to other myelin antigens. The establishment of the apparent autoimmune repertoire against this antigen has been suggested to involve cross-priming during viral infection. Yet, another possibility would be that determinant spreading during ocular inflammation could generate a response to alpha B-crystallin, since it is also a major component of the eye. In this study, we compared serum IgG, IgA and IgM repertoires against a range of eye lens-derived ocular antigens using sera from healthy control subjects and MS patients with or without uveitis. This comparison revealed that among ocular antigens, alpha B-crystallin is the dominant target antigen for serum autoantibodies in both MS patients and healthy controls. Uveitis generally did not affect the antibody reactivity profile. These data provide further support for the notion that a normal adult human immune system is selectively reactive to alpha B-crystallin and they indicate that this responsiveness is unlikely to result from determinant spreading following ocular inflammation. © 2006 Edward Arnold (Publishers) Ltd. Chemicals / CAS: immunoglobulin G, 97794-27-9; immunoglobulin M, 9007-85-6; alpha-Crystallin B Chain; Autoantibodies; Autoantigens; Eye Proteins Subject HealthBiomedical ResearchAlpha B-crystallinDeterminant spreadingAlpha crystallinAutoantibodyImmunoglobulin AImmunoglobulin GImmunoglobulin MAntigen antibody reactionCellular immunityClinical articleControlled studyDisease associationEye inflammationImmune responseImmunoglobulin blood levelImmunoreactivityT lymphocyteAdultalpha-Crystallin B ChainAntibody SpecificityAutoantibodiesAutoantigensChromatography, High Pressure LiquidElectrophoresis, Polyacrylamide GelEye ProteinsFemaleHumansImmune SystemLens, CrystallineMaleMiddle AgedMultiple SclerosisUveitis To reference this document use: http://resolver.tudelft.nl/uuid:a4effa4d-d9e1-40d3-abe3-c6d43518954f DOI https://doi.org/10.1191/135248506ms1271oa TNO identifier 239300 ISSN 1352-4585 Source Multiple Sclerosis, 12 (3), 287-293 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.