Print Email Facebook Twitter Synthesis and biological evaluation of disubstituted N6- cyclopentyladenine analogues: The search for a neutral antagonist with high affinity for the adenosine A1 receptor Title Synthesis and biological evaluation of disubstituted N6- cyclopentyladenine analogues: The search for a neutral antagonist with high affinity for the adenosine A1 receptor Author de Ligt, R.A.F. van der Klein, P.A.M. von Frijtag Drabbe Künzel, J.K. Lorenzen, A. El Maate, F.A. Fujikawa, S. van Westhoven, R. van den Hoven, T. Brussee, J. Ijzerman, A.P. TNO Voeding Publication year 2004 Abstract Novel 3,8- and 8,9-disubstituted N6-cyclopentyladenine derivatives were synthesised in moderate overall yield from 6-chloropurine. The derivatives were made in an attempt to find a new neutral antagonist with high affinity for adenosine A1 receptors. N6-Cyclopentyl-9- methyladenine (N-0840) was used as a lead compound. Binding affinities of the new analogues were determined for human adenosine A1 and A 3 receptors. Their intrinsic activity was assessed in [ 35S]GTPγS binding experiments. Elongation of the 9-methyl of N-0840 to a 9-propyl substituent was very well tolerated. A 9-benzyl group, on the other hand, caused a decrease in adenosine A1 receptor affinity. Next, the 8-position was examined in detail, and affinity was increased with appropriate substitution. Most derivatives were A1-selective and 20 of the new compounds (6-9, 15-21, 23-26, 28, 31, 33, 35, and 36) had higher adenosine A1 receptor affinity than the reference substance, N-0840. Compound 31 (N6-cyclopentyl-8-(N-methylisopropylamino)-9- methyladenine, LUF 5608) had the highest adenosine A1 receptor affinity, 7.7 nM. In the [35S]GTPγS binding experiments, derivatives 5, 14, 22, 23, 25, 26, 33 and 34 did not significantly change basal [35S]GTPγS binding, thus behaving as neutral antagonists. Moreover, four of these compounds (23, 25, 26, and 33) displayed a 4- to 10-fold increased adenosine A1 receptor affinity (75-206 nM) compared to N-0840 (852 nM). In summary, we synthesised a range of N-0840 analogues with higher affinity for adenosine A1 receptors. In addition, four new derivatives, LUF 5666 (23), LUF 5668 (25), LUF 5669 (26) and LUF 5674 (33), behaved as neutral antagonists when tested in [ 35S]GTPγS binding studies. Thus, these compounds have improved characteristics as neutral adenosine A1 receptor antagonists. © 2003 Elsevier Ltd. All rights reserved. Subject Biology ToxicologyPhysiological Sciences[35S]GTPγS bindingAdenosine A1 receptorInverse agonismN-0840 derivativesNeutral antagonist8 bromo 9 methyladenine8 bromo n 6 cyclopentyl 9 methyladenine8 bromo n 6 cyclopentyl 9 propyladenine9 allyl 8 bromo n 6 cyclopentyladenine9 allyl n 6 cyclopentyl 8 methoxyadenineadenine derivativeadenosine A1 receptor antagonistluf 5666luf 5668luf 5669luf 5674n 0840n 6 cyclopentyl 8 (ethylthio) 9 methyladeninen 6 cyclopentyl 8 (n ethyl n butylamino) 9 methyladeninen 6 cyclopentyl 8 (n methyl n ethylamino) 9 methyladeninen 6 cyclopentyl 8 (n methyl n propylamino) 9 methyladeninen 6 cyclopentyl 8 (n methylamino) 9 methyladeninen 6 cyclopentyl 8 (n methylisopropylamino) 9 methyladeninen 6 cyclopentyl 8 (n piperidino) 9 methyladeninen 6 cyclopentyl 8 (n pyrrolidino) 9 methyladeninen 6 cyclopentyl 8 (n,n diethylamino) 9 methyladeninen 6 cyclopentyl 8 ethoxy 9 methyladeninen 6 cyclopentyl 8 isopropoxy 9 methyladeninen 6 cyclopentyl 8 methoxy 9 methyladeninen 6 cyclopentyl 8 methoxy 9 propyladeninen 6 cyclopentyl 9 methyl 8 oxoadeninen 6 cyclopentyl 9 methyl 8 propoxyadeninen 6 cyclopentyl 9 methyladenineunclassified drugarticlebinding affinitydrug determinationdrug synthesisdrug tolerabilityreceptor affinitysubstitution reactionAdenineAdenosineAnimalsCell LineCHO CellsCricetinaeDrug Evaluation, PreclinicalHumansReceptor, Adenosine A1 To reference this document use: http://resolver.tudelft.nl/uuid:a222bf15-678d-4484-b72a-f0fce076b7d3 DOI https://doi.org/10.1016/j.bmc.2003.10.023 TNO identifier 237589 ISSN 0968-0896 Source Bioorganic and Medicinal Chemistry, 12 (1), 139-149 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.