Print Email Facebook Twitter Effect of supplementation with an 80:20 cis9,trans11 conjugated linoleic acid blend on the human platelet proteome Title Effect of supplementation with an 80:20 cis9,trans11 conjugated linoleic acid blend on the human platelet proteome Author Bachmair, E.-M. Bots, M.L. Mennen, L.I. Kelder, T. Evelo, C.T. Horgan, G.W. Ford, I. de Roos, B. Publication year 2012 Abstract Scope: The dietary fatty acid cis9,trans11 conjugated linoleic acid (cis9,trans11 CLA) has been shown to modify the function of endothelial cells, monocytes, and platelets, all of which are involved in the development of atherosclerosis. Potential mechanisms for the platelet effects have not been assessed previously. In this study, we assessed how supplementation of the diet with an 80:20 cis9,trans11 CLA blend affects the platelet proteome. Methods and results: In a double-blind, randomized, placebo-controlled, parallel-group trial, 40 overweight but apparently healthy adults received either 4 g per day of cis9,trans11 CLA-enriched oil or placebo oil, consisting of palm oil and soybean oil, for 3 months. Total platelet proteins were extracted from washed platelets, separated using two-dimensional gel electrophoresis and differentially regulated protein spots were identified by LC-ESI-MS/MS. Supplementation with the CLA blend, compared with placebo, resulted in significant alterations in levels of 46 spots (p < 0.05), of which 40 were identified. Network analysis revealed that the majority of these proteins participate in regulation of the cytoskeleton and platelet structure, as well as receptor action, signaling, and focal adhesion. Conclusion: The platelet proteomics approach revealed novel insights into regulation of cellular biomarkers of atherogenic and thrombotic pathways by an 80:20 cis9,trans11 CLA blend. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Subject LifeMSB - Microbiology and Systems BiologyEELS - Earth, Environmental and Life SciencesFood and NutritionHealthy LivingConjugated linoleic acidCytoscapeDietary supplementation trialPlatelet proteomics To reference this document use: http://resolver.tudelft.nl/uuid:a1312ff4-f69e-4ef1-8ea0-91f8224e4564 DOI https://doi.org/10.1002/mnfr.201100763 TNO identifier 462190 ISSN 1613-4125 Source Molecular Nutrition and Food Research, 56 (7), 1148-1159 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.