Print Email Facebook Twitter Heme biosynthesis and its regulation: Towards understanding and improvement of heme biosynthesis in filamentous fungi Title Heme biosynthesis and its regulation: Towards understanding and improvement of heme biosynthesis in filamentous fungi Author Franken, A.C.W. Lokman, B.C. Ram, A.F.J. Punt, P.J. van den Hondel, C.A.M.J.J. de Weert, S. Publication year 2011 Abstract Heme biosynthesis in fungal host strains has acquired considerable interest in relation to the production of secreted heme-containing peroxidases. Class II peroxidase enzymes have been suggested as eco-friendly replacements of polluting chemical processes in industry. These peroxidases are naturally produced in small amounts by basidiomycetes. Filamentous fungi like Aspergillus sp. are considered as suitable hosts for protein production due to their high capacity of protein secretion. For the purpose of peroxidase production, heme is considered a putative limiting factor. However, heme addition is not appropriate in large-scale production processes due to its high hydrophobicity and cost price. The preferred situation in order to overcome the limiting effect of heme would be to increase intracellular heme levels. This requires a thorough insight into the biosynthetic pathway and its regulation. In this review, the heme biosynthetic pathway is discussed with regards to synthesis, regulation, and transport. Although the heme biosynthetic pathway is a highly conserved and tightly regulated pathway, the mode of regulation does not appear to be conserved among eukaryotes. However, common factors like feedback inhibition and regulation by heme, iron, and oxygen appear to be involved in regulation of the heme biosynthesis pathway in most organisms. Therefore, they are the initial targets to be investigated in Aspergillus niger. © 2011 The Author(s). Subject LifeMSB - Microbiology and Systems BiologyEELS - Earth, Environmental and Life SciencesNutritionAspergillusHeme biosynthesisHeme regulationIntracellular hemePeroxidaseProtein production To reference this document use: http://resolver.tudelft.nl/uuid:a0a7c73b-bb62-4279-9c9e-f834df6373f2 DOI https://doi.org/10.1007/s00253-011-3391-3 TNO identifier 434670 ISSN 0175-7598 Source Applied Microbiology and Biotechnology, 91 (3), 447-460 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.