Title
Strain-dependent induction of cytokine profiles in the gut by orally administered Lactobacillus strains
Author
Maassen, C.B.M.
van Holten-Neelen, C.
Balk, F.
den Bak-Glashouwer, M.-J.H.
Leer, R.J.
Laman, J.D.
Boersma, W.J.A.
Claassen, E.
Publication year
2000
Abstract
Different Lactobacillus strains are frequently used in consumer food products. In addition, recombinant lactobacilli which contain novel expression vectors can now be used in immunotherapeutic applications such as oral vaccination strategies and in T cell tolerance induction approaches for autoimmune disease. Both for food and clinical applications of lactobacilli, proper selection of wild type strains is crucial.For that purpose, eight different common Lactobacillus strains were analysed with respect to mucosal induction of pro- and anti-inflammatory cytokines, IgA-producing plasma cells in the gut, as well as systemic antibody responses against a parenterally administered antigen. Immunohistochemical analysis of cytokine-producing cells in the gut villi showed no significant induction of the cytokines IL-1α, IFN-γ, IL-4 or IL-10 after oral administration of wild type Lactobacillus strains. In contrast, oral administration of L. reuteri and L. brevis induced expression of the proinflammatory/Th1 cytokines TNF-α, IL-2 and/or IL-1β. Oral administration of these two strains and L. fermentum also significantly enhanced the IgG response against parenterally administered haptenated chicken gamma globulin (TNP-CGG). The five other strains did not show this adjuvanticity. L. reuteri induced relatively high levels of IgG2a compared to L. murines, a nonadjuving Lactobacillus strain.These findings imply that different Lactobacillus strains induce distinct mucosal cytokine profiles and possess differential intrinsic adjuvanticity. This suggests that rational Lactobacillus strain selection provides a strategy to influence cytokine expression and thereby influence immune responses. Copyright (C) 2000 Elsevier Science Ltd
Subject
Immunohistochemistry
Mucosal immune response
Probiotic
adjuvant
cytokine
gamma interferon
immunoglobulin A
interleukin 10
interleukin 1alpha
interleukin 1beta
interleukin 2
interleukin 4
probiotic agent
tumor necrosis factor alpha
animal experiment
animal model
antibody response
article
controlled study
cytokine production
enzyme linked immunosorbent assay
female
humoral immunity
immune response
immunohistochemistry
intestine mucosa
Lactobacillus
Lactobacillus brevis
Lactobacillus casei
Lactobacillus fermentum
Lactobacillus plantarum
Lactobacillus reuteri
mouse
mucosa
nonhuman
priority journal
Adjuvants, Immunologic
Administration, Oral
Animals
Chickens
Chikungunya virus
Cytokines
Duodenum
Female
Food Microbiology
gamma-Globulins
Haptens
Injections, Intraperitoneal
Interleukin-2
Intestinal Mucosa
Lactobacillus
Lactobacillus casei
Mice
Mice, Inbred BALB C
Microvilli
Peyer's Patches
Species Specificity
Th2 Cells
Viral Vaccines
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http://resolver.tudelft.nl/uuid:a0877eee-0971-47b5-9e4a-9d1c1c5f626c
TNO identifier
71917
Source
Vaccine, 18, 2613-2623
Document type
article