Title
Ciliated cells in vitamin A-deprived cultured hamster tracheal epithelium do divide
Author
Centraal Instituut voor Voedingsonderzoek TNO
Rutten, A.A.J.J.L.
Beems, R.B.
Wilmer, J.W.G.M.
Feron, V.J.
Publication year
1988
Abstract
The pseudostratified tracheal epithelium, composed of a heterogeneous phenotypically varying cell population, was studied with respect to the in vitro cell proliferative activity of differentiated epithelial cells. Ciliated tracheal epithelial cells so far have been considered to be terminally differentiated, nonproliferating cells. Tracheal organ cultures obtained from vitamin A-deprived Syrian Golden hamsters were cultured in a vitamin A-deficient, serum-free, hormone-supplemented medium. In vitamin A-deprived tracheal epithelium treated with physiologically active all-trans retinol and low cigarette-smoke condensate concentrations it is possible to stimulate the cell proliferation of both basal and columnar cells. Therefore, the probability of finding proliferating columnar cells was increased compared with the in vivo and the vitamin A-deprived situation in which cell proliferative activity is relatively low. In the presence of cigarette-smoke condensate in a noncytotoxic concentration, basal, small mucous granule, ciliated, and indifferent tracheal epithelial cells incorporated [methyl-3H]-thymidine into the DNA during the S phase. The finding that ciliated cells were labeled was supported by serial sections showing the same labeled ciliated cell in two section planes separated by 2 to 3 μm, without labeled epithelial cells next to the ciliated cell. Furthermore, a ciliated tracheal epithelial cell incorporating [methyl-3H]thymidine into DNA was also seen in tracheal cultures of vitamin A-deprived hamsters treated with all-trans retinol in a physiologic concentration. © 1988 Tissue Culture Association, Inc. Chemicals/CAS: retinol, 68-26-8, 82445-97-4; Vitamin A, 11103-57-4
Subject
cell proliferation
cigarette-smoke condensate
ciliated cells
organ culture
tracheal epithelium
vitamin A
retinol
animal
autoradiography
cell culture
cell differentiation
cell division
cytology
drug effect
epithelium
epithelium cell
eukaryotic flagellum
hamster
pathology
plant
retinol deficiency
smoke
tobacco
trachea
Animal
Autoradiography
Cell Differentiation
Cell Division
Cells, Cultured
Cilia
Epithelial Cells
Epithelium
Hamsters
Mesocricetus
Plants, Toxic
Smoke
Support, Non-U.S. Gov't
Tobacco
Trachea
Vitamin A
Vitamin A Deficiency
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http://resolver.tudelft.nl/uuid:a0777595-cdf2-4bd7-9dcf-b7ef9ef907ba
DOI
https://doi.org/10.1007/bf02623905
TNO identifier
230693
ISSN
0883-8364
Source
In Vitro Cellular & Developmental Biology, 24 (24), 931-935
Document type
article