Title
Identification and monitoring of effector and regulatory T cells during experimental arthritis based on differential expression of CD25 and CD134
Author
Nolte-'t Hoen, E.N.M.
Boot, E.P.J.
Wagenaar-Hilbers, J.P.A.
van Bilsen, J.H.M.
Arkesteijn, G.J.A.
Storm, G.
Everse, L.A.
van Eden, W.
Wauben, M.H.M.
TNO Kwaliteit van Leven
Publication year
2008
Abstract
Major problems in the analysis of CD4+ effector cell and regulatory T cell (Treg) populations in an activated immune system are caused by the facts that both cell types can express CD25 and that the discriminatory marker forkhead box p3 can only be analyzed in nonviable (permeabilized) cells. Here, we show that CD134 (OX40) can be used as a discriminatory marker combined with CD25 to isolate and characterize viable CD4+ effector cells and Tregs. Before and during adjuvant arthritis in rats, coexpression of CD134 and CD25 identified activated Tregs consistently, as these T cells proliferated poorly to disease-associated antigens and were suppressive in vitro and in vivo. Depending on the time of isolation and location, CD4+ T cell populations expressing CD134 or CD25 contained effector/memory T cells. Analysis of the function, phenotype, and amount of the CD4+ T cell subsets in different lymph node stations revealed spatiotemporal differences in effector cell and Treg compartments during experimental arthritis. © Society for Leukocyte Biology.
Subject
Biology
FoxP3
Immune regulation
Kinetics
Lymph nodes
CD134 antigen
interleukin 2 receptor alpha
transcription factor FOXP3
animal cell
animal experiment
animal model
arthritis
article
CD25+ T lymphocyte
CD4+ T lymphocyte
cell activation
cell differentiation
cell proliferation
controlled study
effector cell
lymph node
male
memory T lymphocyte
nonhuman
phenotype
priority journal
protein analysis
protein expression
protein function
protein isolation
protein localization
rat
regulatory T lymphocyte
Animals
Arthritis, Experimental
Disease Models, Animal
Disease Progression
Forkhead Transcription Factors
Interleukin-2 Receptor alpha Subunit
Male
Rats
Rats, Inbred Lew
Receptors, OX40
T-Lymphocytes, Regulatory
Rattus
To reference this document use:
http://resolver.tudelft.nl/uuid:9e3d93dc-6950-4d07-a2f5-ac199ebd6ec3
DOI
https://doi.org/10.1189/jlb.0607436
TNO identifier
240605
ISSN
0741-5400
Source
Journal of Leukocyte Biology, 83 (1), 112-121
Document type
article