Print Email Facebook Twitter Apolipoprotein CI inhibits scavenger receptor BI and increases plasma HDL levels in vivo Title Apolipoprotein CI inhibits scavenger receptor BI and increases plasma HDL levels in vivo Author de Haan, W. Out, R. Berbée, J.F.P. van der Hoogt, C.C. Dijk, K.W.v. van Berkel, T.J.C. Romijn, J.A. Wouter Jukema, J. Havekes, L.M. Rensen, P.C.N. TNO Kwaliteit van Leven Publication year 2008 Abstract Apolipoprotein CI (apoCI) has been suggested to influence HDL metabolism by activation of LCAT and inhibition of HL and CETP. However, the effect of apoCI on scavenger receptor BI (SR-BI)-mediated uptake of HDL-cholesteryl esters (CE), as well as the net effect of apoCI on HDL metabolism in vivo is unknown. Therefore, we evaluated the effect of apoCI on the SR-BI-mediated uptake of HDL-CE in vitro and determined the net effect of apoCI on HDL metabolism in mice. Enrichment of HDL with apoCI dose-dependently decreased the SR-BI-dependent association of [3H]CE-labeled HDL with primary murine hepatocytes, similar to the established SR-BI-inhibitors apoCIII and oxLDL. ApoCI deficiency in mice gene dose-dependently decreased HDL-cholesterol levels. Adenovirus-mediated expression of human apoCI in mice increased HDL levels at a low dose and increased the HDL particle size at higher doses. We conclude that apoCI is a novel inhibitor of SR-BI in vitro and increases HDL levels in vivo. © 2008 Elsevier Inc. All rights reserved. Subject HealthPhysiological SciencesHigh density lipoproteinOxidized LDLSR-BITransgenic micecholesterol esterhigh density lipoprotein cholesterolscavenger receptor BIAdenovirusanimal cellcholesterol metabolismdose responsein vivo studyliver cellmousenonhumanparticle sizeprotein expressionAdenoviridaeAnimalsApolipoprotein C-ICholesterol, HDLGene Transfer TechniquesHepatocytesHumansMiceMice, KnockoutScavenger Receptors, Class BAdenoviridaeMurinaeMusMus musculus To reference this document use: http://resolver.tudelft.nl/uuid:9b69fd32-d47c-4fd5-9b5c-883ea34fa10c DOI https://doi.org/10.1016/j.bbrc.2008.10.147 TNO identifier 241309 ISSN 0006-291X Source Biochemical and Biophysical Research Communications, 377 (4), 1294-1298 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.