Print Email Facebook Twitter Interactions between polycyclic aromatic hydrocarbons in binary mixtures: Effects on gene expression and DNA adduct formation in precision-cut rat liver slices Title Interactions between polycyclic aromatic hydrocarbons in binary mixtures: Effects on gene expression and DNA adduct formation in precision-cut rat liver slices Author Staal, Y.C.M. Pushparajah, D.S. van Herwijnen, M.H.M. Gottschalk, R.W.H. Maas, L.M. Ioannides, C. van Schooten, F.J. van Delft, J.H.M. TNO Kwaliteit van Leven Publication year 2008 Abstract Although exposure to polycyclic aromatic hydrocarbons (PAHs) occurs mostly through mixtures, hazard and risk assessment are mostly based on the effects caused by individual compounds. The objective of the current study was to investigate whether interactions between PAHs occur, focusing on gene expression (as measured by cDNA microarrays) and DNA adduct formation. The effects of benzo[a]pyrene or dibenzo[a,h]anthracene (DB[a,h]A) alone and in binary mixtures with another PAH (DB[a,h]A, benzo[b]fluoranthene, fluoranthene or dibenzo[a,l]pyrene) were investigated using precision-cut rat liver slices. All compounds significantly modulated the expression of several genes, but overlap between genes affected by the mixture and by the individual compounds was relatively small. All mixtures showed an antagonistic response on total gene expression profiles. Moreover, at the level of individual genes, mostly antagonism was evident, with additivity and synergism observed for only a few genes. As far as DNA adduct formation is concerned, the binary mixtures generally caused antagonism. The effects in liver slices suggest a lower carcinogenic potency of PAH mixtures than estimated based on additivity of individual compounds. © The Author 2008. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. Subject Nutritionbenzo[a]pyrenebenzo[b]fluoranthenecomplementary DNAdibenz[a,h]anthracenedibenzo[a,l]pyrenefluoranthenepolycyclic aromatic hydrocarbonanimal experimentanimal tissuearticlecarcinogenic activityDNA adductDNA microarraygene expressiongene interactionliver slicemalemolecular interactionnonhumannucleotide sequencepriority journalratAnimalsCarcinogensDNA AdductsGene ExpressionLiverMaleOligonucleotide Array Sequence AnalysisPolycyclic Hydrocarbons, AromaticRatsRats, WistarRattus To reference this document use: http://resolver.tudelft.nl/uuid:9a9067d3-19ac-4331-8003-b2cf777eb101 DOI https://doi.org/10.1093/mutage/gen041 TNO identifier 241098 ISSN 0267-8357 Source Mutagenesis, 23 (6), 491-499 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.