Title
A generic model for glucose production from various cellulose sources by a commercial cellulase complex
Author
Drissen, R.E.T.
Maas, R.H.W.
van der Maarel, M.J.E.C.
Kabel, M.A.
Schols, H.A.
Tramper, J.
Beeftink, H.H.
TNO Kwaliteit van Leven
Publication year
2007
Abstract
The kinetics of cellulose hydrolysis by commercially available Cellubrix were described mathematically, with Avicel and wheat straw as substrates. It was demonstrated that hydrolysis could be described by three reactions: direct glucose formation and indirect glucose formation via cellobiose. Hydrolysis did not involve any soluble oligomers apart from low amounts of cellobiose. Phenomena included in the mathematical model were substrate limitation, adsorption of enzyme onto substrate, glucose inhibition, temperature dependency of reaction rates, and thermal enzyme inactivation. In addition, substrate heterogeneity was described by a recalcitrance constant. Model parameters refer to both enzyme characteristics and substrate-specific characteristics. Quantitative model development was carried out on the basis of Avicel hydrolysis. In order to describe wheat straw hydrolysis, wheat straw specific parameter values were measured. Updating the pertinent parameters for wheat straw yielded a satisfactory description of wheat straw hydrolysis, thus underlining the generic potential of the model. Chemicals / CAS: cellobiose, 16462-44-5, 528-50-7; cellulose, 61991-22-8, 68073-05-2, 9004-34-6; glucose, 50-99-7, 84778-64-3; microcrystalline cellulose, 39394-43-9, 51395-75-6
Subject
Health
Cellulose
Glucose
Model
Adsorption
Cellulose
Enzyme inhibition
Hydrolysis
Mathematical models
Oligomers
Reaction rates
Cellobiose
Enzyme inactivation
Glucose formation
Glucose
Triticum aestivum
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DOI
https://doi.org/10.1080/10242420701510668
TNO identifier
240261
ISSN
1024-2422
Source
Biocatalysis and Biotransformation, 25 (6), 419-429
Document type
article