Title
Effects of vitamin C supplementation in human volunteers with a range of cholesterol levels on biomarkers of oxygen radical-generated damage
Author
Anderson, D.
Phillips, B.J.
Yu, T-W.
Edwards, A.J.
Ayesh, R.
Butterworth, K.R.
Publication year
2000
Abstract
Twenty-four men and 24 women, all nonsmoking, and maintaining normal dietary habits were assigned to 3 groups of 16. Each group comprising 4 males with "low" cholesterol levels (<6 mmol/L) matched for age and build with 4 males with "high" cholesterol levels (>6 mmol/L) and 8 similarly matched females. A three-treatment, three-treatment period, cross-over design was adopted. The three treatments were placebo, 60 mg vitamin C/day (the recommended daily allowance) and 6 g vitamin C/day for 14 days with 6 weeks between treatments. Blood samples were taken at the end of each treatment period. Vitamin C supplementation significantly increased plasma vitamin C concentrations and total antioxidant capacity, but did not affect cholesterol status or plasma ras p21 protein levels. There was a nonsignificant dose-related decrease in plasma lipid peroxidation breakdown products. DNA damage, measured in lymphocytes by the Comet assay and chromosome aberration test, was not increased after vitamin C supplementation. Sensitivity to hydrogen peroxide (in the Comet assay) was also unaffected, but sensitivity to chromosome aberration induced by bleomycin was increased by supplementation. A significant gender difference was found in plasma vitamin C levels, antioxidant capacity, and number of chromosome aberrations. Results were independent of low and high cholesterol status.
Subject
Medicine
Geneeskunde
Health
Gezondheid
Dietetics
Voedingsleer
ascorbic acid
biological marker
bleomycin
cholesterol
horlicks
oxygen radical
placebo
protein p21
Ras protein
sanatogen
adult
aged
antioxidant activity
cholesterol blood level
chromosome aberration
clinical trial
comet assay
conference paper
controlled clinical trial
controlled study
crossover procedure
DNA damage
dose response
female
human
human experiment
lipid peroxidation
lymphocyte
male
normal human
oxidative stress
priority journal
sex difference
vitamin blood level
vitamin supplementation
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http://resolver.tudelft.nl/uuid:97bb85fc-61be-4d65-9181-a59aecefabf7
TNO identifier
41673
Source
Pure and Applied Chemistry, 72 (6), 973-983
Document type
article