Title
Lipidome Analysis in Brain and Peripheral Plasma Following Milk Fat Globule Membrane Supplementation in Rodents
Author
Davies, R.
van Diepen, J.A.
Brink, L.R.
Bijlsma, S.
Neufeld, K.A.M.
Cryan, J.F.
O'Mahony, S.M.
Bobeldijk, I.
Gross, G.
Publication year
2022
Abstract
Milk fat globule membrane (MFGM) is an essential component of milk. Bovine MFGM (bMFGM) has been shown to support cognitive development and increase relative concentrations of serum phospholipids. This study investigates bioavailability of bMFGM components after oral administration in two preclinical models to explore whether dietary bMFGM induces parallel changes to plasma and brain lipidomes. Methods and results. Transgenic APOE*3.Leiden mice (n = 18 per group) and Sprague–Dawley rats (n = 12 per group) are fed bMFGM-enriched (MFGM+) or Control diet, followed by phospholipid profile-determination in plasma, hippocampus, and prefrontal cortex tissue by targeted mass spectrometry. Multivariate analysis of lipidomic profiles demonstrates a separation between MFGM+ and Control plasma across rodents. In plasma, sphingomyelins contributed the most to the separation of lipid patterns among both models, where three sphingomyelins (d18:1/14:0, d18:1/23:0, d18:1/23:1[9Z]) are consistently higher in the circulation of MFGM+ groups. A similar trend is observed in rat prefrontal cortex, although no significant separation of the brain lipidome is demonstrated. Conclusion. bMFGM-enriched diet alters plasma phospholipid composition in rodents, predominantly increasing sphingomyelin levels in the systemic circulation with similar, but non-significant, trends in central brain regions. These changes may contribute to the beneficial effects of bMFGM on neurodevelopment during early life.
Subject
Blood plasma
Brain
Lipidomics
Milk fat globule membrane
Rodents
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http://resolver.tudelft.nl/uuid:96532be6-7fdc-4c54-bf59-7a90c035518e
DOI
https://doi.org/10.1002/mnfr.202200177
TNO identifier
977355
ISSN
1613-4125
Source
Molecular Nutrition and Food Research, 66 (66)
Document type
article