Effect of retinol and cigarette-smoke and condensate on dye-coupled intercellular communication between hamster tracheal epithelial cells
de Haan, L.H.J.
Centraal Instituut voor Voedingsonderzoek TNO
The dye-coupled intercellular communication across gap junctions in primary hamster tracheal epithelial cells has been studied in serum-free, hormone-supplemented medium. In the absence of vitamin A, non-cytotoxic concentrations of cigarette-smoke condensate (CSC) inhibited intercellular communication between tracheal epithelial cells in a concentration-dependent way. All-trans retinol and retinoic acid showed biphasic effects on intercellular communication depending on their concentration. Physiological concentrations of retinol and retinoic acid increased the dye-coupled transfer of Lucifer Yellow CH via gap junctions compared with the dimethylsulfoxide-treated tracheal epithelial cells. At pharmacological concentrations retinol slightly increased the intercellular communication in the first 2 h of the exposure period, whereas upon longer treatment times with retinol and retinoic acid, gap-junction-mediated intercellular communication was inhibited almost completely. When retinol was given to tracheal epithelial cells before exposure to CSC or simultaneously with CSC-exposure, retinol counteracted the inhibitory potential of CSC on intercellular communication. The results of the present study clearly indicate that both CSC and all-trans retinol influence the intercellular communication betwen primary hamster tracheal epithelial cells in serum-free, hormone-supplemented culture medium. Chemicals/CAS: lucifer yellow, 67769-47-5, 82446-52-4; retinoic acid, 302-79-4; retinol, 68-26-8, 82445-97-4; Dimethyl Sulfoxide, 67-68-5; Fluorescent Dyes; Isoquinolines; lucifer yellow, 77944-88-8; Vitamin A, 11103-57-4
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animal cell cell junction
Support, Non-U.S. Gov't
Carcinogenesis, 9 (2), 315-320