Title
Effect of retinol and cigarette-smoke and condensate on dye-coupled intercellular communication between hamster tracheal epithelial cells
Author
Rutten, A.A.J.J.L.
Jongen, W.M.F.
de Haan, L.H.J.
Hendriksen, E.G.J.
Koeman, J.H.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
1988
Abstract
The dye-coupled intercellular communication across gap junctions in primary hamster tracheal epithelial cells has been studied in serum-free, hormone-supplemented medium. In the absence of vitamin A, non-cytotoxic concentrations of cigarette-smoke condensate (CSC) inhibited intercellular communication between tracheal epithelial cells in a concentration-dependent way. All-trans retinol and retinoic acid showed biphasic effects on intercellular communication depending on their concentration. Physiological concentrations of retinol and retinoic acid increased the dye-coupled transfer of Lucifer Yellow CH via gap junctions compared with the dimethylsulfoxide-treated tracheal epithelial cells. At pharmacological concentrations retinol slightly increased the intercellular communication in the first 2 h of the exposure period, whereas upon longer treatment times with retinol and retinoic acid, gap-junction-mediated intercellular communication was inhibited almost completely. When retinol was given to tracheal epithelial cells before exposure to CSC or simultaneously with CSC-exposure, retinol counteracted the inhibitory potential of CSC on intercellular communication. The results of the present study clearly indicate that both CSC and all-trans retinol influence the intercellular communication betwen primary hamster tracheal epithelial cells in serum-free, hormone-supplemented culture medium. Chemicals/CAS: lucifer yellow, 67769-47-5, 82446-52-4; retinoic acid, 302-79-4; retinol, 68-26-8, 82445-97-4; Dimethyl Sulfoxide, 67-68-5; Fluorescent Dyes; Isoquinolines; lucifer yellow, 77944-88-8; Vitamin A, 11103-57-4
Subject
cigarette smoke
lucifer yellow
radioisotope
retinoic acid
retinol
animal cell cell junction
cytochemistry
cytology
female
male
nonhuman
trachea
ultrastructure
Animal
Cell Communication
Cells, Cultured
Dimethyl Sulfoxide
Epithelial Cells
Epithelium
Fluorescent Dyes
Hamsters
Isoquinolines
Mesocricetus
Mucociliary Clearance
Plants, Toxic
Smoke
Support, Non-U.S. Gov't
Tobacco
Trachea
Vitamin A
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TNO identifier
230538
ISSN
0143-3334
Source
Carcinogenesis, 9 (2), 315-320
Document type
article