Print Email Facebook Twitter The influence of treatment in alpine and moderate maritime climate on the composition of the skin microbiome in patients with difficult to treat atopic dermatitis Title The influence of treatment in alpine and moderate maritime climate on the composition of the skin microbiome in patients with difficult to treat atopic dermatitis Author van Mierlo, M.M.F. Totté, J.E.E. Fieten, K.B. van den Broek, T.J. Schuren, F.H.J. Pardo, L.M. Pasmans, S.G.M.A. Publication year 2019 Abstract Background: The skin microbiome, characterized by an overgrowth of Staphylococcus aureus, plays an important role in the pathogenesis of atopic dermatitis (AD). Multidisciplinary treatment in alpine climate is known for its positive effect on disease severity in children with AD and can result in a different immune response compared with moderate maritime climate. However, the effect on the composition of the skin microbiome in AD is unknown. Objective: To determine the effect of treatment in alpine climate and moderate maritime climate on the microbiome for lesional and non-lesional skin in children with difficult to treat AD. Results: Alpine climate treatment led to a significant change in the microbiota on lesional skin, whereas no significant change was found after moderate maritime climate. On both lesional and non-lesional skin, we observed a significant increase in Shannon diversity and a significant decrease in both Staphylococcus abundance and S aureus load after alpine climate treatment. The decrease in S aureus was significantly larger on lesional skin following alpine climate treatment compared with moderate maritime climate treatment. Staphylococcus epidermidis load was stable over time. Conclusions and clinical relevance: Alpine climate treatment leads to significant changes in the composition of the skin microbiome in children with AD, mainly caused by a reduction in the Staphylococcus genus. This study shows new perspectives in the potential mode of action for therapies in AD. © 2019 John Wiley & Sons Ltd Subject Atopic dermatitis;DermatologyMicrobiomePaediatricsStaphylococcus To reference this document use: http://resolver.tudelft.nl/uuid:938b0dbc-9cda-4c79-8e9f-65f48e6e9a11 DOI https://doi.org/10.1111/cea.13492 TNO identifier 870070 ISSN 0954-7894 Source Clinical and Experimental Allergy Document type article Files To receive the publication files, please send an e-mail request to TNO Library.