Print Email Facebook Twitter An Advanced LC–MS/MS Platform for the Analysis of Specialized Pro-Resolving Lipid Mediators Title An Advanced LC–MS/MS Platform for the Analysis of Specialized Pro-Resolving Lipid Mediators Author Jónasdóttir, H.S. Ioan-Facsinay, A. Kwekkeboom, J. Brouwers, H. Zuurmond, A.M. Toes, R. Deelder, A.M. Giera, M. Publication year 2014 Abstract Here we present an advanced platform for the analysis of specialized pro-resolving mediators (SPM), including a set of pro-inflammatory lipids and respective biochemical pathway markers. The platform is characterized by a largely simplified sample preparation protocol, employing methanol protein-precipitation instead of solid phase extraction. Sample preparation and analysis can be carried out in 96-well format, ensuring higher throughput. In addition, faster analysis times and higher sensitivities have been achieved when compared to other platforms. In total 36 lipid mediators—SPM, their pathway markers and a set of pro-inflammatory lipids including three internal standards—are analyzed within an 11 min LC–MS/MS run. The method was validated using human plasma, including repeatability, linearity, recovery, matrix effects, and accuracy. The presented platform was applied in the analysis of synovial fluid post-mortem samples and cellular extracts from polymorphonuclear cells as well as whole blood treated with ionophore in the presence of the polyunsaturated fatty acid eicosapentaenoic acid. © 2014, Springer-Verlag Berlin Heidelberg. Subject LifeMHR - Metabolic Health ResearchELSS - Earth, Life and Social SciencesBiomedical InnovationBiologyHealthy LivingEPAInflammation resolutionLC–MSLTE4Pro-resolving mediatorsCell extractIcosapentaenoic acidIonophoreLipidMethanolPolyunsaturated fatty acidSpecialized pro resolving lipidUnclassified drugIsolation and purificationLipid analysisLiquid chromatographyMass spectrometryPolymorphonuclear cellSolid phase extractionSynovial fluidValidation study To reference this document use: http://resolver.tudelft.nl/uuid:933c73ae-8e3d-44c8-a889-06d690dcf52e DOI https://doi.org/10.1007/s10337-014-2779-5 TNO identifier 524069 ISSN 0009-5893 Source Chromatographia, 78 (5-6), 391-401 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.