Print Email Facebook Twitter Binding and transfer of verocytotoxin by polymorphonuclear leukocytes in hemolytic uremic syndrome Title Binding and transfer of verocytotoxin by polymorphonuclear leukocytes in hemolytic uremic syndrome Author te Loo, D.M.W.M. Monnens, L.A.H. van der Velden, T.J.A.M. Vermeer, M.A. Preyers, F. Demacker, P.N.M. van den Heuvel, L.P.W.J. van Hinsbergh, V.W.M. Gaubius instituut TNO Publication year 2000 Abstract The hemolytic uremic syndrome (HUS) is the most common cause of acute renal failure in children. The role of a verocytotoxin (VT)-producing Escherichia coli has been strongly implicated in the epidemic form of HUS. Although direct toxicity of VT on glomerular endothelial cells has been demonstrated, it remained still unclear how the VT is transported from the intestine to the target organs. In this study we demonstrate that VT, when incubated in whole blood, binds rapidly and completely to human polymorphonuclear leukocytes (PMNs) and not to other components of blood. Binding studies with 125I-VT-1 showed a single class of binding sites on freshly isolated, non-stimulated human PMNs. The K(d) of VT-binding to PMNs was 10-8 mol/L, 100-fold less than that of the VT-receptor globotriaosylceramide. On incubation of VT-preloaded PMNs with human glomerular microvascular endothelial cells (GMVECs), transfer of VT-1 to the endothelial cells occurred. Incubation of non-stimulated GMVECs with VT- preloaded PMNs, but not with PMNs or VT-1 alone, caused inhibition of protein synthesis and cell death. Our data are in concert with a role of PMNs in the transfer of VT from the intestine to the kidney endothelium. This transfer occurs by selective binding to a specific receptor on PMNs and subsequent passing of the ligand VT to the VT-receptor on GMVECs, which causes cell damage. This new mechanism further underpins the important role of PMNs in HUS. (C) 2000 by The American Society of Hematology. Subject BiologyAdultBacterial ToxinsChildEndothelium, VascularEscherichia coliFluorescein-5-isothiocyanateHemolytic-Uremic SyndromeHumansIodine RadioisotopesKidney GlomerulusKineticsLipoproteinsLipoproteins, HDLLipoproteins, LDLLipoproteins, VLDLMicrocirculationNeutrophilsReceptors, Cell SurfaceShiga-Like Toxin ITrihexosylceramides To reference this document use: http://resolver.tudelft.nl/uuid:9312e315-4421-45ab-a5e5-62fb039b71f7 TNO identifier 235574 ISSN 0006-4971 Source Blood, 95 (11), 3396-3402 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.