Title
Construction and heterologous expression of a synthetic copy of the cutinase cDNA from Fusarium solani pisi
Author
van Gemeren, I.A.
Musters, W.
van den Hondel, C.A.M.J.J.
Verrips, C.T.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
1995
Abstract
A copy of the cutinase cDNA from Fusarium solani pisi was constructed starting from synthetic oligonucleotides. For this construction three separate cassettes were made, which were subsequently assembled to form the cutinase gene. Heterologous expression of the synthetic cutinase gene and the subsequent secretion of the recombinant enzyme was achieved in Saccharomyces cerevisiae and Aspergillus awamori.
Subject
Nutrition
A. awamori
F. solani pisi cutinase
Filamentous fungi
Heterologous expression
S. cerevisiae
Synthetic gene construction
Complementary dna
Cutinase
Aspergillus
Biosynthesis
Controlled study
Fusarium solani
Gene expression
Nonhuman
Priority journal
Saccharomyces cerevisiae
Amino Acid Sequence
Aspergillus
Base Sequence
Biotechnology
Carboxylic Ester Hydrolases
DNA, Complementary
DNA, Fungal
Fusarium
Genes, Fungal
Genes, Synthetic
Genetic Vectors
Molecular Sequence Data
Plasmids
Recombinant Proteins
Saccharomyces cerevisiae
Support, Non-U.S. Gov't
Aspergillus
Aspergillus awamori
Fungi
Fusarium
Fusarium solani
Saccharomyces cerevisiae
To reference this document use:
http://resolver.tudelft.nl/uuid:91d8e5c4-e538-42f8-b106-1dfe3695197d
DOI
https://doi.org/10.1016/0168-1656(95)00041-n
TNO identifier
83418
Source
Journal of Biotechnology, 40, 155-162
Document type
article