Print Email Facebook Twitter Construction and heterologous expression of a synthetic copy of the cutinase cDNA from Fusarium solani pisi Title Construction and heterologous expression of a synthetic copy of the cutinase cDNA from Fusarium solani pisi Author van Gemeren, I.A. Musters, W. van den Hondel, C.A.M.J.J. Verrips, C.T. Centraal Instituut voor Voedingsonderzoek TNO Publication year 1995 Abstract A copy of the cutinase cDNA from Fusarium solani pisi was constructed starting from synthetic oligonucleotides. For this construction three separate cassettes were made, which were subsequently assembled to form the cutinase gene. Heterologous expression of the synthetic cutinase gene and the subsequent secretion of the recombinant enzyme was achieved in Saccharomyces cerevisiae and Aspergillus awamori. Subject NutritionA. awamoriF. solani pisi cutinaseFilamentous fungiHeterologous expressionS. cerevisiaeSynthetic gene constructionComplementary dnaCutinaseAspergillusBiosynthesisControlled studyFusarium solaniGene expressionNonhumanPriority journalSaccharomyces cerevisiaeAmino Acid SequenceAspergillusBase SequenceBiotechnologyCarboxylic Ester HydrolasesDNA, ComplementaryDNA, FungalFusariumGenes, FungalGenes, SyntheticGenetic VectorsMolecular Sequence DataPlasmidsRecombinant ProteinsSaccharomyces cerevisiaeSupport, Non-U.S. Gov'tAspergillusAspergillus awamoriFungiFusariumFusarium solaniSaccharomyces cerevisiae To reference this document use: http://resolver.tudelft.nl/uuid:91d8e5c4-e538-42f8-b106-1dfe3695197d DOI https://doi.org/10.1016/0168-1656(95)00041-n TNO identifier 83418 Source Journal of Biotechnology, 40, 155-162 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.