Print Email Facebook Twitter Quantifying phenotypic flexibility as the response to a high-fat challenge test in different states of metabolic health Title Quantifying phenotypic flexibility as the response to a high-fat challenge test in different states of metabolic health Author Kardinaal, A.F.M. van Erk, M.J. Dutman, A.E. Stroeve, J.H.M. van de Steeg, E. Bijlsma, S. Kooistra, T. van Ommen, B. Wopereis, S. Publication year 2015 Abstract Metabolism maintains homeostasis at chronic hypercaloric conditions, activating postprandial response mechanisms, which come at the cost of adaptation processes such as energy storage, eventually with negative health consequences. This study quantified the metabolic adaptation capacity by studying challenge response curves. After a high-fat challenge, the 8 h response curves of 61 biomarkers related to adipose tissue mass and function, systemic stress, metabolic flexibility, vascular health, and glucose metabolism was compared between 3 metabolic health stages: 10 healthy men, before and after 4 wk of highfat, high-calorie diet (1300 kcal/d extra), and 9 men with metabolic syndrome (MetS). The MetS subjects had increased fasting concentrations of biomarkers representing the 3 core processes, glucose, TG, and inflammation control, and the challenge response curves ofmost biomarkers were altered. After the 4 wk hypercaloric dietary intervention, these 3 processes were not changed, as compared with the preintervention state in the healthy subjects, whereas the challenge response curves of almost all endocrine, metabolic, and inflammatory processes regulating these core processes were altered, demonstrating major molecular physiologic efforts tomaintain homeostasis.This study thus demonstrates that change in challenge response is a more sensitive biomarker of metabolic resilience than are changes in fasting concentrations. Chemicals/CAS: glucose, 50-99-7, 84778-64-3 Subject LifeMSB - Microbiology and Systems BiologyELSS - Earth, Life and Social SciencesBiomedical InnovationBiologyHealthy LivingClinical studyEicosanoidsHuman physiologyNutritionSystems biologyFatGlucoseAdipose tissueAdultAgedControlled studyGlucose metabolismHealthHomeostasisHumanMaleMetabolic syndrome XMetabolismOpen studyPilot studyProvocation testQuantitative analysisStress To reference this document use: http://resolver.tudelft.nl/uuid:91720e82-28ad-484c-a606-7fe750a3b434 DOI https://doi.org/10.1096/fj.14-269852 TNO identifier 530274 ISSN 0892-6638 Source FASEB Journal, 29 (11), 4600-4613 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.