Title
Differential expression of stress proteins in human adult astrocytes in response to cytokines
Author
Bajramović, J.J.
Bsibsi, M.
Geutskens, S.B.
Hassankhan, R.
Verhulst, K.C.
Stege, G.J.J.
de Groot, C.J.A.
van Noort, J.M.
TNO Preventie en Gezondheid
Publication year
2000
Abstract
Various lines of evidence suggest a close relationship between heat shock proteins (hsp) and several autoimmune diseases such as arthritis, diabetes and multiple sclerosis. While enhanced expression of hsp in autoimmune diseases is often regarded as a non-specific bystander effect of the inflammatory process, surprisingly little is known on hsp regulation by inflammatory mediators such as cytokines. In this study cytokine-induced expression of hsp60, hsp27 and αB-crystallin was studied in cultures of primary human adult astrocytes at the mRNA as well as at the protein level. We show differential hsp expression patterns in response to pro-inflammatory and immunoregulatory cytokines. Hsp60 expression was found to be enhanced in response to cytokines as diverse as IL-1β, TNF-α, IL-4, IL-6 and IL-10. Upregulation of hsp27, however, was primarily induced by immunoregulatory cytokines like IL-4, IL-6 and TGF-β whereas αB-crystallin expression was found to be enhanced by the pro-inflammatory cytokine TNF-α only. None of the cytokines studied was able to enhance expression of all three hsp simultaneously. These results show that in human astrocytes induced expression of hsp27 and αB-crystallin is dependent on the presence of a defined set of stimuli, while induced expression of hsp60 is a much less selective event. This highly differential pattern of hsp expression in response to inflammatory mediators known to play an important role in the pathogenesis of autoimmune diseases indicates that hsp responses are specific rather than non-specific bystander responses. (C) 2000 Elsevier Science B.V. Chemicals/CAS: Adjuvants, Immunologic; Chaperonin 60; Crystallins; Cytokines; Heat-Shock Proteins; Inflammation Mediators; RNA, Messenger
Subject
Health
Astrocytes
Heat shock proteins
Human
Multiple sclerosis
Heat shock protein
Heat shock protein 27
Heat shock protein 60
Interleukin 10
Interleukin 1beta
Interleukin 4
Interleukin 6
Messenger RNA
Tumor necrosis factor alpha
Adult
Autoimmune disease
Human cell
Immunoregulation
Inflammation
Protein expression
Adjuvants, Immunologic
Aged
Aged, 80 and over
Astrocytes
Cells, Cultured
Chaperonin 60
Crystallins
Cytokines
Female
Heat-Shock Proteins
Humans
Inflammation Mediators
Male
Middle Aged
RNA, Messenger
Up-Regulation
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http://resolver.tudelft.nl/uuid:90c6030d-710a-41f1-a92a-f2d713745bc3
DOI
https://doi.org/10.1016/s0165-5728(99)00260-x
TNO identifier
235602
ISSN
0165-5728
Source
Journal of Neuroimmunology, 106 (1-2), 14-22
Document type
article