Title
Transcription factor NF-kB as a potential biomarker for oxidative stress
Author
Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding
van den Berg, R.
Haenen, G.R.M.M.
van den Berg, H.
Bast, A.
Publication year
2001
Abstract
There is increasing interest in the involvement of transcription factors, such as of the transcription factor NF-κB (nuclear factor-κB), in the pathogenesis of various diseases. NF-κB is involved in the control of the transcription of a variety of cellular genes that regulate the inflammatory response by the production of cytokines, chemokines, cell adhesion molecules and acute phase proteins. The involvement of NF-κB is especially of interest as it is activated by oxidative stress and its activation can be modulated by antioxidant compounds. The activation of NF-κB can be determined by the electromobility shift assay (EMSA) with a NF-κB binding-site-specific probe. EMSA can also be used on human mononuclear cells isolated from peripheral blood, which could make the assay applicable for clinical trials. The critical steps of the EMSA are discussed, addressing some pitfalls of the assay. The procedure that can be used to express NF-κB activity in human subjects is evaluated. This offers the possibility to use NF-κB as a functional biomarker of oxidative stress as illustrated by several examples of in vitro and in vivo studies. Chemicals/CAS: Biological Markers; NF-kappa B
Subject
Biological Markers
Cell Line
Cell Nucleus
Electrophoresis, Polyacrylamide Gel
Hela Cells
Humans
Leukocytes, Mononuclear
NF-kappa B
Oxidative Stress
Sarcoidosis
Antioxidants
Reactive oxygen species
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TNO identifier
57159
ISSN
0007-1145
Source
British Journal of Nutrition, 86 (86), S121-S127
Document type
article