Print Email Facebook Twitter Effect of cisplatin exposure on platinum accumulation and growth inhibition in human neoplastic and normal squamous epithelial cells of the mucosa of the upper-aerodigestive tract Title Effect of cisplatin exposure on platinum accumulation and growth inhibition in human neoplastic and normal squamous epithelial cells of the mucosa of the upper-aerodigestive tract Author Braakhuis, B.J.M. Welters, M.J.P. Cloos, J. Pankras, J.E. Smeets, S.J. Fichtinger-Schepman, A.-M.J. Centraal Instituut voor Voedingsonderzoek TNO Publication year 1999 Abstract The aim of the present study was to investigate how normal head and neck epithelial cells (NHNEC) respond to cisplatin compared to their neoplastic counterparts with respect to intracellular platinum (Pt) levels and growth inhibition. A colorimetric assay was used to assess growth inhibition after exposure to cisplatin for 72 h. Growth inhibition did not differ between cultures of neoplastic (n=5) and normal cells (n=5). Intracellular Pt levels, determined with atomic absorption spectroscopy were about 30-fold higher in the normal epithelial cells. The main finding of this study is that normal epithelial cells from the head and neck region have a much higher tolerance for cisplatin than their neoplastic counterparts. Interestingly, this characteristic is without consequence for growth inhibition. Copyright (C) 1999 Elsevier Science Ireland Ltd. Subject NutritionCisplatinHead and neckKeratinocytesNon-malignant cellsPt accumulationSquamous cellsCisplatinPlatinumAdultAntineoplastic activityCancer cell cultureColorimetryControlled studyDrug accumulationEpithelium cellFemaleGrowth inhibitionHead and neck cancerHumanHuman cellKeratinocyteMalePalatePriority journalSquamous cellSquamous epitheliumAntineoplastic AgentsCarcinoma, Squamous CellCell DivisionCisplatinEpithelial CellsFemaleHead and Neck NeoplasmsHumansMaleMiddle AgedPlatinumTumor Cells, CulturedUvula To reference this document use: http://resolver.tudelft.nl/uuid:8f7807c1-4f53-45f5-80ae-7a36892539eb DOI https://doi.org/10.1016/s0304-3835(99)00016-6 TNO identifier 235012 ISSN 0304-3835 Source Cancer Letters, 138 (1-2), 217-220 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.