Title
Effect of cisplatin exposure on platinum accumulation and growth inhibition in human neoplastic and normal squamous epithelial cells of the mucosa of the upper-aerodigestive tract
Author
Braakhuis, B.J.M.
Welters, M.J.P.
Cloos, J.
Pankras, J.E.
Smeets, S.J.
Fichtinger-Schepman, A.-M.J.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
1999
Abstract
The aim of the present study was to investigate how normal head and neck epithelial cells (NHNEC) respond to cisplatin compared to their neoplastic counterparts with respect to intracellular platinum (Pt) levels and growth inhibition. A colorimetric assay was used to assess growth inhibition after exposure to cisplatin for 72 h. Growth inhibition did not differ between cultures of neoplastic (n=5) and normal cells (n=5). Intracellular Pt levels, determined with atomic absorption spectroscopy were about 30-fold higher in the normal epithelial cells. The main finding of this study is that normal epithelial cells from the head and neck region have a much higher tolerance for cisplatin than their neoplastic counterparts. Interestingly, this characteristic is without consequence for growth inhibition. Copyright (C) 1999 Elsevier Science Ireland Ltd.
Subject
Nutrition
Cisplatin
Head and neck
Keratinocytes
Non-malignant cells
Pt accumulation
Squamous cells
Cisplatin
Platinum
Adult
Antineoplastic activity
Cancer cell culture
Colorimetry
Controlled study
Drug accumulation
Epithelium cell
Female
Growth inhibition
Head and neck cancer
Human
Human cell
Keratinocyte
Male
Palate
Priority journal
Squamous cell
Squamous epithelium
Antineoplastic Agents
Carcinoma, Squamous Cell
Cell Division
Cisplatin
Epithelial Cells
Female
Head and Neck Neoplasms
Humans
Male
Middle Aged
Platinum
Tumor Cells, Cultured
Uvula
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DOI
https://doi.org/10.1016/s0304-3835(99)00016-6
TNO identifier
235012
ISSN
0304-3835
Source
Cancer Letters, 138 (1-2), 217-220
Document type
article