Title
Preferential campesterol incorporation into various tissues in apolipoprotein E*3-Leiden mice consuming plant sterols or stanols
Author
Plat, J.
de Jong, A.
Volger, O.L.
Princen, H.M.G.
Mensink, R.P.
TNO Kwaliteit van Leven
Publication year
2008
Abstract
Intestinal absorption of plant sterols and stanols is much lower as compared with that of cholesterol; and therefore, serum concentrations are low. Circulating plant sterols and stanols are incorporated into tissues. However, hardly any data are available about tissue distributions of individual plant sterols and stanols, particularly in relation to their serum concentrations. We therefore fed female apolipoprotein E*3-Leiden mice a control diet, a plant sterol-enriched diet (1g/100 g diet), or a plant stanol-enriched diet (1g/100 g diet) for 8 weeks. In the sterol group, serum cholesterol-standardized campesterol and sitosterol concentrations were, respectively, 8 and 7 times higher as compared with those in the control group. Consequently, the serum campesterol-sitosterol ratio remained essentially unchanged. Cholesterol-standardized plant sterol concentrations increased significantly in all analyzed tissues, except brain. However, the campesterol-sitosterol ratio also increased in all tissues (except in liver and spleen), suggesting that campesterol is preferentially incorporated over sitosterol in those tissues. For the stanol group, serum plant stanol concentrations also increased; but the increase was but less pronounced. We conclude that, in apolipoprotein E*3-Leiden mice, campesterol is preferentially incorporated into most tissues over sitosterol, which cannot be deduced from changes in serum concentrations. © 2008 Elsevier Inc. All rights reserved.
Subject
Biology
Biomedical Research
apolipoprotein E3
campesterol
cholesterol
phytosterol
sitosterol
stanol ester
animal experiment
animal tissue
article
concentration (parameters)
controlled study
diet supplementation
female
mouse
nonhuman
priority journal
tissue distribution
Animals
Apolipoprotein E3
Cholesterol
Diet
Female
Mice
Mice, Transgenic
Osmolar Concentration
Phytosterols
Sitosterols
Tissue Distribution
To reference this document use:
http://resolver.tudelft.nl/uuid:8d345951-55e3-4565-b7f3-8d5392f52258
DOI
https://doi.org/10.1016/j.metabol.2008.04.018
TNO identifier
240974
ISSN
0026-0495
Source
Metabolism: Clinical and Experimental, 57 (9), 1241-1247
Document type
article