Print Email Facebook Twitter Extracellular matrix components and regulators in the airway smooth muscle in asthma Title Extracellular matrix components and regulators in the airway smooth muscle in asthma Author Araujo, B.B. Dolhnikoff, M. Silva, L.F.F. Elliot, J. Lindeman, J.H.N. Ferreira, D.S. Mulder, A. Gomes, H.A.P. Fernezlian, S.M. James, A. Mauad, T. TNO Kwaliteit van Leven Publication year 2008 Abstract There is an intimate relationship between the extracellular matrix (ECM) and smooth muscle cells within the airways. Few studies have comprehensively assessed the composition of different ECM components and its regulators within the airway smooth muscle (ASM) in asthma. With the aid of image analysis, the fractional areas of total collagen and elastic fibres were quantified within the ASM of 35 subjects with fatal asthma (FA) and compared with 10 nonfatal asthma (NFA) patients and 22 nonasthmatic control cases. Expression of collagen I and III, fibronectin, versican, matrix metalloproteinase (MMP)-1, -2, -9 and -12 and tissue inhibitor of metalloproteinase-1 and -2 was quantified within the ASM in 22 FA and 10 control cases. In the large airways of FA cases, the fractional area of elastic fibres within the ASM was increased compared with NFA and controls. Similarly, fibronectin, MMP-9 and MMP-12 were increased within the ASM in large airways of FA cases compared with controls. Elastic fibres were increased in small airways in FA only in comparison with NFA cases. There is altered extracellular matrix composition and a degradative environment within the airway smooth muscle in fatal asthma patients, which may have important consequences for the mechanical and synthetic functions of airway smooth muscle. Copyright©ERS Journals Ltd 2008. Chemicals / CAS: collagen, 9007-34-5; fibronectin, 86088-83-7; gelatinase A, 146480-35-5; gelatinase B, 146480-36-6; interstitial collagenase, 9001-12-1; tissue inhibitor of metalloproteinase 1, 140208-24-8; tissue inhibitor of metalloproteinase 2, 124861-55-8; versican, 126968-45-4; tissue inhibitor of metalloproteinase, 97837-28-0; Matrix Metalloproteinases, EC 3.4.24.-; Tissue Inhibitor of Metalloproteinases Subject Airway smooth muscleExtracellular matrixFatal asthmaMetalloproteinasesRemodellingTissue inhibitor of metalloproteinaseAdolescentAdultAsthmaBronchiCase-Control StudiesExtracellular MatrixFemaleHumansMaleMatrix MetalloproteinasesMiddle AgedMuscle, SmoothTissue Inhibitor of Metalloproteinases To reference this document use: http://resolver.tudelft.nl/uuid:8cecc08a-4d37-4c50-8d83-8a490fdcc8a8 DOI https://doi.org/10.1183/09031936.00147807 TNO identifier 240901 ISSN 0903-1936 Source European Respiratory Journal, 32 (1), 61-69 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.