Print Email Facebook Twitter Comparison of six commercial ELISA kits for their specificity and sensitivity in detecting different major peanut allergens Title Comparison of six commercial ELISA kits for their specificity and sensitivity in detecting different major peanut allergens Author Jayasena, S. Smits, M. Fiechter, D. de Jong, A. Nordlee, J. Baumert, J. Taylor, S.L. Pieters, R.H. Koppelman, S.J. Publication year 2015 Abstract Six commercial peanut enzyme-linked immunosorbent assay kits were assessed for their ability to recover peanut from the standard reference material 2387 peanut butter and also for their specificity in detecting four major peanut allergens, Ara h 1, Ara h 2, Ara h 3, and Ara h 6. The percentage recovery of peanut from peanut butter differed across different kits as well as at different sample concentrations. The highest recovery was observed with the Romer and R-Biopharm kits, while four other kits were found to underestimate the protein content of the reference peanut butter samples. Five of the kits were most sensitive in detecting Ara h 3 followed by Ara h 1, while hardly recognizing Ara h 2 and Ara h 6. The other kit showed the highest sensitivity to Ara h 2 and Ara h 6, while Ara h 1 and Ara h 3 were poorly recognized. Although Ara h 2 and Ara h 6 are known to be heat stable and more potent allergens, antisera specific to any of these four peanut proteins/allergens may serve as good markers for the detection of peanut residues. © 2015 American Chemical Society. Subject LifeFI - Functional IngredientsELSS - Earth, Life and Social SciencesBiomedical InnovationBiologHealthy LivingDetectionAllergensError detectionFood productsProteinsRecoveryAra h 1Ara h 2Ara h 3Ara h 6ELISAImmunoassayPeanutOilseedsAraArachis hypogaea To reference this document use: http://resolver.tudelft.nl/uuid:8ce16bf6-5996-492f-a46f-d8363a037c1a DOI https://doi.org/10.1021/jf504741t TNO identifier 524128 ISSN 0021-8561 Source Journal of Agricultural and Food Chemistry, 63 (6), 1849-1855 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.