Title
Gastrointestinal AAPOAII and systemic AA-amyloidosis in aged C57BL/Ka mice. Amyloid-type dependent effect of long-term immunosuppressive treatment
Author
Gezondheidsorganisatie TNO Instituut voor verouderings- en vaatziekten onderzoek TNO
HogenEsch, H.
Niewold, T.A.
Higuchi, K.
Tooten, P.C.J.
Gruys, E.
Radl, J.
Publication year
1993
Abstract
The light microscopic and immunohistochemical features of a novel localized senile amyloidosis in the gastrointestinal tract of C57BL/Ka mice are described. Senile gastrointestinal amyloidosis was predominantly found in the lamina propria of the ileum, cecum and stomach and infrequently in other segments of the gastrointestinal tract. The Congo red affinity of the senile amyloid was sensitive to potassium permanganate pretreatment. The amyloid did not react with anti-AA and anti-immunoglobulin antisera, but stained positively for apoAII, a major apolipoprotein of high density lipoproteins. A similar type of amyloid, termed AApoAII, has recently been described in a systemic form of senile amyloidosis in mice. In the present study, we investigated the effect of long-term immunosuppressive treatment on the incidence of systemic AA-amyloidosis and gastrointestinal AApoAII-amyloidosis in aged C57BL/Ka mice. Gastrointestinal amyloidosis occurred in 60% of the control mice, but significantly less in mice of the immunosuppressed groups. In contrast, systemic AA-immunoreactive amyloidosis was only found in mice that were given immunosuppressive treatment. There was no codeposition of AA and AApoAII-amyloid. These findings indicate that immunosuppressive drugs have a profound effect on the incidence as well as the type of amyloidosis in C57BL/Ka mice. Chemicals/CAS: amyloid A protein, 59165-71-8; congo red, 573-58-0, 80701-77-5; permanganate potassium, 7722-64-7; Amyloid Protein AA; Apolipoproteins A; Immunosuppressive Agents
Subject
Amyloidosis
Apolipoprotein AII
Immunosuppression
Intestine
Mice
amyloid a protein
congo red
high density lipoprotein
lipoprotein a
permanganate potassium
amyloidosis
animal experiment
animal tissue
article
cecum
controlled study
female
gastrointestinal tract
ileum
immunohistochemistry
immunosuppressive treatment
lamina propria
microscopy
morbidity
mouse
nonhuman
priority journal
senescence
stomach
Aging
Amyloid Protein AA
Amyloidosis
Animal
Apolipoproteins A
Blotting, Western
Electrophoresis, Polyacrylamide Gel
Female
Gastrointestinal Diseases
Immunoenzyme Techniques
Immunosuppressive Agents
Incidence
Mice
Mice, Inbred C57BL
Support, Non-U.S. Gov't
Time Factors
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DOI
https://doi.org/10.1007/bf02915094
TNO identifier
232135
ISSN
0340-6075
Source
Virchows Archiv - B Cell Pathology Including Molecular Pathology, 64 (64), 37-43
Document type
article