Print Email Facebook Twitter Gastrointestinal AAPOAII and systemic AA-amyloidosis in aged C57BL/Ka mice. Amyloid-type dependent effect of long-term immunosuppressive treatment Title Gastrointestinal AAPOAII and systemic AA-amyloidosis in aged C57BL/Ka mice. Amyloid-type dependent effect of long-term immunosuppressive treatment Author Gezondheidsorganisatie TNO Instituut voor verouderings- en vaatziekten onderzoek TNO HogenEsch, H. Niewold, T.A. Higuchi, K. Tooten, P.C.J. Gruys, E. Radl, J. Publication year 1993 Abstract The light microscopic and immunohistochemical features of a novel localized senile amyloidosis in the gastrointestinal tract of C57BL/Ka mice are described. Senile gastrointestinal amyloidosis was predominantly found in the lamina propria of the ileum, cecum and stomach and infrequently in other segments of the gastrointestinal tract. The Congo red affinity of the senile amyloid was sensitive to potassium permanganate pretreatment. The amyloid did not react with anti-AA and anti-immunoglobulin antisera, but stained positively for apoAII, a major apolipoprotein of high density lipoproteins. A similar type of amyloid, termed AApoAII, has recently been described in a systemic form of senile amyloidosis in mice. In the present study, we investigated the effect of long-term immunosuppressive treatment on the incidence of systemic AA-amyloidosis and gastrointestinal AApoAII-amyloidosis in aged C57BL/Ka mice. Gastrointestinal amyloidosis occurred in 60% of the control mice, but significantly less in mice of the immunosuppressed groups. In contrast, systemic AA-immunoreactive amyloidosis was only found in mice that were given immunosuppressive treatment. There was no codeposition of AA and AApoAII-amyloid. These findings indicate that immunosuppressive drugs have a profound effect on the incidence as well as the type of amyloidosis in C57BL/Ka mice. Chemicals/CAS: amyloid A protein, 59165-71-8; congo red, 573-58-0, 80701-77-5; permanganate potassium, 7722-64-7; Amyloid Protein AA; Apolipoproteins A; Immunosuppressive Agents Subject AmyloidosisApolipoprotein AIIImmunosuppressionIntestineMiceamyloid a proteincongo redhigh density lipoproteinlipoprotein apermanganate potassiumamyloidosisanimal experimentanimal tissuearticlececumcontrolled studyfemalegastrointestinal tractileumimmunohistochemistryimmunosuppressive treatmentlamina propriamicroscopymorbiditymousenonhumanpriority journalsenescencestomachAgingAmyloid Protein AAAmyloidosisAnimalApolipoproteins ABlotting, WesternElectrophoresis, Polyacrylamide GelFemaleGastrointestinal DiseasesImmunoenzyme TechniquesImmunosuppressive AgentsIncidenceMiceMice, Inbred C57BLSupport, Non-U.S. Gov'tTime Factors To reference this document use: http://resolver.tudelft.nl/uuid:8c5f2a6f-bc3b-47d1-8adc-2d54fd56fe4d DOI https://doi.org/10.1007/bf02915094 TNO identifier 232135 ISSN 0340-6075 Source Virchows Archiv - B Cell Pathology Including Molecular Pathology, 64 (64), 37-43 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.