Title
Docosahexaenoic acid and eicosapentaenoic acid are converted by 3T3-L1 adipocytes to N-acyl ethanolamines with anti-inflammatory properties
Author
Balvers, M.G.J.
Verhoeckx, K.C.M.
Plastina, P.
Wortelboer, H.M.
Meijerink, J.
Witkamp, R.F.
TNO Kwaliteit van Leven
Publication year
2010
Abstract
n-3 PUFAs have beneficial health effects which are believed to be partly related to their anti-inflammatory properties, however the exact mechanisms behind this are unknown. One possible explanation could be via their conversion to N-acyl ethanolamines (NAEs), which are known to possess anti-inflammatory properties. Using fatty acid precursors we showed that 3T3-L1 adipocytes are indeed able to convert docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) to their NAE derivatives docosahexaenoyl ethanolamine (DHEA) and eicosapentaenoyl ethanolamine (EPEA), respectively. This synthesis took place on top of an apparent background formation of these NAEs in standard culture medium. In addition we were able to demonstrate the presence of DHEA, but not of EPEA, in human plasma. DHEA and EPEA were found to decrease LPS induced adipocyte IL-6 and MCP-1 levels. Results of combined incubations with PPAR-γ and CB2 antagonists suggest a role of these receptors in mediating the reduction of IL-6 by DHEA. Our results are in line with the hypothesis that in addition to other pathways, formation of N-acyl ethanolamines may contribute to the biological activity of n-3 PUFAs. Different targets, including the endocannabinoid system, may be involved in the immune-modulating activity of these "fish-oil-derived NAEs." © 2010 Elsevier B.V.
Subject
Biology
Biomedical Research
Adipocytes
DHA
EPA
Inflammation
N-acyl ethanolamines
docosahexaenoic acid
docosahexaenoyl ethanolamine
eicosapentanaenoyl ethanolamine
icosapentaenoic acid
interleukin 6
lipopolysaccharide
monocyte chemotactic protein 1
n acyl ethanolamine
unclassified drug
antiinflammatory activity
article
cell strain 3T3
enzyme structure
enzyme synthesis
nonhuman
priority journal
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http://resolver.tudelft.nl/uuid:8b5f096b-4514-403e-98d9-38c6395ad9ac
TNO identifier
409226
ISSN
1388-1981
Source
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1801 (10), 1107-1114
Document type
article