Title
Immunoelectronmicroscopy of the binding of high density lipoprotein apoproteins to normal and hypercholesterolemic human fibroblasts
Author
Emeis, J.J.
Vermeer, B.J.
Havekes, L.
Gaubius instituut TNO
Publication year
1981
Abstract
We visualized the apolipoproteins of high density lipoproteins (HDL) that were bound to cultured human fibroblasts at the ultrastructural level, with an immunoenzymecytochemical method. By using antisera against (a) apolipoprotein AI (a major apolipoprotein of HDL) and (b) apolipoprotein E (an apolipoprotein present in HDL), the binding of HDL at 4??C to indentations of the plasma membrane could be demonstrated. The binding of HDL to fibroblasts obtained from a patient homozygous for familial hypercholesterolemia proved to be indistinguishable from the binding to normal fibroblasts. Chemicals/CAS: Apolipoprotein A-I; apolipoprotein E-A-II; Apolipoproteins; Lipoproteins, HDL
Subject
Biology
Apolipoprotein A-I
Apolipoproteins
Cell Membrane
Cells, Cultured
Fibroblasts
Human
Hypercholesterolemia, Familial
Immunologic Techniques
Lipoproteins, HDL
Microscopy, Electron
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TNO identifier
228974
ISSN
0309-1651
Source
Cell Biology International Reports, 5 (2), 133-141
Document type
article