Title
Induction of somatic mutations but not methylated DNA adducts in λlacZ transgenic mice by dichlorvos
Author
Pletsa, V.
Steenwinkel, M.-J.S.T.
van Delft, J.H.M.
Baan, R.A.
Kyrtopoulos, S.A.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
1999
Abstract
In order to examine the in vivo genotoxic activity of dichlorvos, λlacZ transgenic mice (Muta(TM)Mouse) were treated i.p. with single (4.4 or 11 mg/kg) or multiple (5x11 mg/kg) doses of this agent and sacrificed 4 h or 14 days post-treatment for DNA adduct measurement or mutant frequency analysis, respectively. Neither methylated DNA adducts nor an increase in mutant frequency were detected in the bone marrow, white blood cells, liver, spleen, lung, brain and sperm cells after the single doses. However, following multiple dosing a statistically significant 3-fold increase in mutant frequency was observed in the liver, while a non-statistically significant increase was observed in the bone marrow. In contrast, dimethylsulphate, a model methylating agent, gave rise to detectable DNA adducts but no increase in mutant frequency following i.p. administration of single (30 mg/kg) or multiple (10x6 mg/kg) doses. Copyright (C) 1999 Elsevier Science Ireland Ltd.
Subject
Nutrition
Dimethylsulphate
Methylating agents
N7-Methylguanine
O6-Methylguanine
Organosphosphate insecticides
6 o methylguanine
7 methylguanine
Dichlorvos
Dimethyl sulfate
Animal cell
Bone marrow cell
Controlled study
DNA adduct
DNA methylation
Genotoxicity
Liver cell
Mouse
Mutation rate
Nonhuman
Priority journal
Somatic mutation
Transgenic mouse
Animals
Dichlorvos
DNA Methylation
Guanine
Insecticides
Lac Operon
Mice
Mice, Transgenic
Mutation
Mus musculus
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http://resolver.tudelft.nl/uuid:8acfed4d-6a40-468a-ac88-61836bc3afce
DOI
https://doi.org/10.1016/s0304-3835(99)00256-6
TNO identifier
235216
ISSN
0304-3835
Source
Cancer Letters, 146 (2), 155-160
Document type
article