Print Email Facebook Twitter Quantitative analysis of Tenecteplase in rat plasma samples using LC-MS/MS as an alternative for ELISA Title Quantitative analysis of Tenecteplase in rat plasma samples using LC-MS/MS as an alternative for ELISA Author Buscher, B.A.P. Gerritsen, H. van Schöll, I. Cnubben, N.H.P. Brüll, L.P. TNO Kwaliteit van Leven Publication year 2007 Abstract An LC-MS/MS method has been developed for the quantitative determination of a protein drug (Tenecteplase; MW 58,777 Da) in rat plasma. The protein was digested with trypsin without prior clean-up of the plasma sample, without the use of a label nor internal standard. A limited validation was performed to assess the linearity, the sensitivity and the specificity of the method. In addition, the developed method was applied to the quantitative analysis of Tenecteplase in rat plasma samples originating from a single-dose study in rats. © 2007 Elsevier B.V. All rights reserved. Subject BiologyAnalytical researchDigestionLiquid chromatographyMass spectrometryPlasmaProteinsQuantitative analysisBloodDigestive systemDrug productsEnzymesSensitivity analysisInternal standardTenecteplaseTrypsinChemical analysistenecteplaseanimal experimentarticleblood samplingcontrolled studydrug distributionenzyme linked immunosorbent assayliquid chromatographymass spectrometrynonhumanpriority journalquantitative analysisratsingle drug doseAnimalsChromatography, LiquidEnzyme-Linked Immunosorbent AssayFibrinolytic AgentsRatsSensitivity and SpecificityTandem Mass SpectrometryTissue Plasminogen ActivatorRattus To reference this document use: http://resolver.tudelft.nl/uuid:8a8f41ec-3377-4b41-8d93-9f3ccd529b87 DOI https://doi.org/10.1016/j.jchromb.2006.12.053 TNO identifier 240016 ISSN 1570-0232 Source Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 852 (1-2), 631-634 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.