Title
Quantitative analysis of Tenecteplase in rat plasma samples using LC-MS/MS as an alternative for ELISA
Author
Buscher, B.A.P.
Gerritsen, H.
van Schöll, I.
Cnubben, N.H.P.
Brüll, L.P.
TNO Kwaliteit van Leven
Publication year
2007
Abstract
An LC-MS/MS method has been developed for the quantitative determination of a protein drug (Tenecteplase; MW 58,777 Da) in rat plasma. The protein was digested with trypsin without prior clean-up of the plasma sample, without the use of a label nor internal standard. A limited validation was performed to assess the linearity, the sensitivity and the specificity of the method. In addition, the developed method was applied to the quantitative analysis of Tenecteplase in rat plasma samples originating from a single-dose study in rats. © 2007 Elsevier B.V. All rights reserved.
Subject
Biology
Analytical research
Digestion
Liquid chromatography
Mass spectrometry
Plasma
Proteins
Quantitative analysis
Blood
Digestive system
Drug products
Enzymes
Sensitivity analysis
Internal standard
Tenecteplase
Trypsin
Chemical analysis
tenecteplase
animal experiment
article
blood sampling
controlled study
drug distribution
enzyme linked immunosorbent assay
liquid chromatography
mass spectrometry
nonhuman
priority journal
quantitative analysis
rat
single drug dose
Animals
Chromatography, Liquid
Enzyme-Linked Immunosorbent Assay
Fibrinolytic Agents
Rats
Sensitivity and Specificity
Tandem Mass Spectrometry
Tissue Plasminogen Activator
Rattus
To reference this document use:
http://resolver.tudelft.nl/uuid:8a8f41ec-3377-4b41-8d93-9f3ccd529b87
DOI
https://doi.org/10.1016/j.jchromb.2006.12.053
TNO identifier
240016
ISSN
1570-0232
Source
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 852 (1-2), 631-634
Document type
article