Print Email Facebook Twitter Does skin prick test reactivity to purified allergens correlate with clinical severity of peanut allergy? Title Does skin prick test reactivity to purified allergens correlate with clinical severity of peanut allergy? Author Peeters, K.A.B.M. Koppelman, S.J. van Hoffen, E. van der Tas, C.W.H. den Hartog Jager, C.F. Penninks, A.H. Hefle, S.L. Bruijnzeel-Koomen, C.A.F.M. Knol, E.F. Knulst, A.C. TNO Kwaliteit van Leven Publication year 2007 Abstract Background: Recognition of specific peanut allergens or the diversity of IgE binding to peanut allergens may play a role in the elicitation of severe allergic reactions. Objective: To investigate whether sensitization to individual allergens Ara h 1, Ara h 2, Ara h 3 and Ara h 6 is correlated with clinical severity. Methods: The reactivity of purified peanut allergens was measured by skin prick test (SPT) and by IgE immunoblot in 30 patients. The results were related to the clinical reactivity by history, and in 25 of them to the eliciting dose (ED). Results: The majority of patients recognized Ara h 2 and Ara h 6. Patients with severe symptoms had a higher SPT response to Ara h 2 and Ara h 6 at low concentrations (0.1 μg/mL) and to Ara h 1 and Ara h 3 at higher concentrations (100 μg/mL), compared with patients with mild symptoms. They also recognized a greater number of allergens and showed a higher cumulative SPT response compared with patients with mild symptoms. No significant differences were observed between patients with a low or high ED. Conclusions: Ara h 2 and Ara h 6 appeared to be more potent than Ara h 1 and Ara h 3. Both SPT reactivity to low concentrations of Ara h 2 and Ara h 6 and to higher concentrations of Ara h 1 and Ara h 3 were shown to be indicative of severe symptoms. © 2007 Blackwell Publishing Ltd. Subject HealthFood technologyAra h 1Ara h 2Ara h 3Ara h 6DBPCFCEliciting doseIgE-immunoblotPeanut allergyPurified allergenSPTallergenadolescentadultagedallergic reactionarticleclinical articlecontrolled studydisease severityfemalehumanimmunoblottingmalepeanut allergyprick testpriority journalskin sensitizationsymptomAdolescentAdultAgedAllergensDose-Response Relationship, ImmunologicDouble-Blind MethodFemaleGlycoproteinsHumansImmunoglobulin EMaleMiddle AgedNo-Observed-Adverse-Effect LevelPeanut AgglutininPeanut HypersensitivityPlant ProteinsPredictive Value of TestsSkinSkin TestsStatistics, Nonparametric To reference this document use: http://resolver.tudelft.nl/uuid:89e0e4e0-e2c6-48f7-9501-11524a5df685 DOI https://doi.org/10.1111/j.1365-2222.2006.02628.x TNO identifier 239818 ISSN 0954-7894 Source Clinical and Experimental Allergy, 37 (1), 108-115 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.