Print Email Facebook Twitter Quantitative profiling of bile acids in biofluids and tissues based on accurate mass high resolution LC-FT-MS: Compound class targeting in a metabolomics workflow Title Quantitative profiling of bile acids in biofluids and tissues based on accurate mass high resolution LC-FT-MS: Compound class targeting in a metabolomics workflow Author Bobeldijk, I. Hekman, M. van der Vries de- Weij, J. Coulier, L. Ramaker, R. Kleemann, R. Kooistra, T. Rubingh, C. Freidig, A. Verheij, E. TNO Kwaliteit van Leven Publication year 2008 Abstract We report a sensitive, generic method for quantitative profiling of bile acids and other endogenous metabolites in small quantities of various biological fluids and tissues. The method is based on a straightforward sample preparation, separation by reversed-phase high performance liquid-chromatography mass spectrometry (HPLC-MS) and electrospray ionisation in the negative ionisation mode (ESI-). Detection is performed in full scan using the linear ion trap Fourier transform mass spectrometer (LTQ-FTMS) generating data for many (endogenous) metabolites, not only bile acids. A validation of the method in urine, plasma and liver was performed for 17 bile acids including their taurine, sulfate and glycine conjugates. The method is linear in the 0.01-1 μM range. The accuracy in human plasma ranges from 74 to 113%, in human urine 77 to 104% and in mouse liver 79 to 140%. The precision ranges from 2 to 20% for pooled samples even in studies with large number of samples (n > 250). The method was successfully applied to a multi-compartmental APOE*3-Leiden mouse study, the main goal of which was to analyze the effect of increasing dietary cholesterol concentrations on hepatic cholesterol homeostasis and bile acid synthesis. Serum and liver samples from different treatment groups were profiled with the new method. Statistically significant differences between the diet groups were observed regarding total as well as individual bile acid concentrations. © 2008 Elsevier B.V. All rights reserved. Subject Analytical researchAccurate massBile acidsBody fluidsHigh performance liquid chromatographyMetabolomicsTargeted metabolite profilingTissuesAcidsArsenic compoundsChlorine compoundsCholesterolChromatographic analysisComplexationElectrospray ionizationFourier transformsHigh performance liquid chromatographyHistologyIonizationIonization of liquidsLiquid chromatographyLiquid phase epitaxyLiverMass spectrometersMass spectrometryMetabolitesPhase separationPlasmasSeparationSpectrometersSpectrometrySulfate mineralsAccurate massBile acid synthesisBile acidsBio-fluidsBiological fluidsCholesterol homeostasisChromatography-mass spectrometryElectro sprayingFourier Transform Mass SpectrometerGeneric methodsHigh resolutionsHPLC-MSHuman plasmasHuman urineLinear ion trapMetabolomicsMouse liverPooled samplesReversed-phase high performanceSample preparationsTargeted metabolite profilingTissuesBody fluidsBile acidGlycineSulfateAccuracyAnimal experimentAnimal tissueArticleBile acid synthesisBody fluidCholesterol intakeCholesterol liver levelControlled studyElectrosprayFourier transform mass spectrometryHomeostasisHumanLiverMetaboliteMetabolomicsNonhumanPriority journalQuantitative analysisReversed phase high performance liquid chromatographyStatistical significanceTissueAnimalsBile Acids and SaltsCholesterol, DietaryChromatography, LiquidComputational BiologyFourier AnalysisHumansLiverMass SpectrometryMetabolismMiceReproducibility of Results To reference this document use: http://resolver.tudelft.nl/uuid:88983458-5ccf-481e-a93d-6613152f7766 DOI https://doi.org/10.1016/j.jchromb.2008.05.008 TNO identifier 240956 ISSN 1570-0232 Source Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 871 (2), 306-313 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.