Title
Profiling the Secretion of Soluble Mediators by End Stage Osteoarthritis Synovial Tissue Explants Reveals a Reduced Responsiveness to an Inflammatory Trigger
Author
Gierman, L.M.
van El, B.
van der Ham, F.
Koudijs, A.
Stoop, R.
Verheijen, J.H.
Kloppenburg, M.
van Osch, G.J.V.M.
Stojanovic-Susulic, V.
Huizinga, T.W.J.
Zuurmond, A.M.
Publication year
2013
Abstract
Objective:Evidence is accumulating that synovial tissue plays an active role in osteoarthritis (OA), however, exact understanding of its contribution is lacking. In order to further elucidate its role in the OA process, we aimed to identify the secretion pattern of soluble mediators by synovial tissue and to assess its ability to initiate cartilage degeneration.Methods:Synovial tissue explants (STEs) obtained from donors without history of OA (n = 8) or from end stage OA patients (n = 16) were cultured alone or together with bovine cartilage explants in the absence or presence of IL-1alpha. The secretion of 48 soluble mediators was measured and the effect on glycosaminoglycan (GAG) release and matrix metalloproteinase (MMP) activity was determined.Results:Normal and OA STEs secreted comparable levels of almost all measured soluble mediators. However, in the presence of IL-1alpha these mediators were less secreted by OA than by normal STEs of which 15 differed significantly (p
Subject
EELS - Earth, Environmental and Life Sciences
Life
Healthy Living
Biomedical Innovation
Biology
MHR - Metabolic Health Research
To reference this document use:
http://resolver.tudelft.nl/uuid:86f7f31c-86b8-41b9-8f0c-106bb11abdb4
DOI
https://doi.org/10.1371/journal.pone.0062634
TNO identifier
472001
ISSN
1932-6203
Source
PLoS ONE, 8 (8)
Document type
article