Print Email Facebook Twitter Complications in the determination of HDL2/HDL3 ratios Title Complications in the determination of HDL2/HDL3 ratios Author Kremer, J.M.H. Havekes, L. Gaubius instituut TNO Publication year 1981 Abstract We investigated different procedures to subfractionate high density lipoprotein (HDL) into its density subfractions (HDL2 and HDL3). When pore-limit as well as zone polyacrylamide gel electrophoresis was used, it proved to be necessary to use freshly prepared and plasma protein-free HDL preparations. For a quantitative subfractionation of HDL in HDL2 and HDL3 we also tested density gradient ultracentrifugation. To avoid interference with LDL contamination a low starting density was required. Both methods, electrophoresis as well as density gradient ultracentrifugation were too laborious and time-consuming for large scale use. We devised a combination of both ultracentrifugation and electrophoresis as a method which is relatively appropriate for determinations of HDL2/HDL3 ratios on large scale. Chemicals/CAS: high density lipoprotein-2; high density lipoprotein-3; Lipoproteins, HDL Subject High density lipoproteinCentrifugation, Density GradientElectrophoresis, Polyacrylamide GelHumanLipoproteins, HDL To reference this document use: http://resolver.tudelft.nl/uuid:8678efc7-0a4c-4c2a-98cb-36dd6191c2c0 DOI https://doi.org/10.1016/0009-8981(81)90132-7 TNO identifier 228976 ISSN 0009-8981 Source Clinica Chimica Acta, 109 (1), 21-29 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.