Title
Complications in the determination of HDL2/HDL3 ratios
Author
Kremer, J.M.H.
Havekes, L.
Gaubius instituut TNO
Publication year
1981
Abstract
We investigated different procedures to subfractionate high density lipoprotein (HDL) into its density subfractions (HDL2 and HDL3). When pore-limit as well as zone polyacrylamide gel electrophoresis was used, it proved to be necessary to use freshly prepared and plasma protein-free HDL preparations. For a quantitative subfractionation of HDL in HDL2 and HDL3 we also tested density gradient ultracentrifugation. To avoid interference with LDL contamination a low starting density was required. Both methods, electrophoresis as well as density gradient ultracentrifugation were too laborious and time-consuming for large scale use. We devised a combination of both ultracentrifugation and electrophoresis as a method which is relatively appropriate for determinations of HDL2/HDL3 ratios on large scale. Chemicals/CAS: high density lipoprotein-2; high density lipoprotein-3; Lipoproteins, HDL
Subject
High density lipoprotein
Centrifugation, Density Gradient
Electrophoresis, Polyacrylamide Gel
Human
Lipoproteins, HDL
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DOI
https://doi.org/10.1016/0009-8981(81)90132-7
TNO identifier
228976
ISSN
0009-8981
Source
Clinica Chimica Acta, 109 (1), 21-29
Document type
article