Print Email Facebook Twitter Heterogeneity of rat liver parenchyma in cholesterol 7α-hydroxylase and bile acids synthesis Title Heterogeneity of rat liver parenchyma in cholesterol 7α-hydroxylase and bile acids synthesis Author Gezondheidsorganisatie TNO Gaubius Instituut TNO Ugele, B. Kempen, J.M. Gebhardt, R. Mijer, P. Burger, H.J. Princen, H.M.G. Publication year 1991 Abstract Periportal and perivenous hepatocytes were isolated from rat liver by digitonin/collagenase perfusion for investigating the acinar distribution of bile acid synthesis. The specific activity of cholesterol 7 alpha-hydroxylase (EC 184.108.40.206) was 7.9-fold higher in perivenous cells than in periportal hepatocytes. Mass production of bile acids differed 4.4-fold between cultured perivenous and periportal hepatocytes. In contrast, the levels of free cholesterol in homogenates and microsomes derived from both subfractions were similar. Feeding of rats with the bile-acid-sequestering anion-exchange resin colestid resulted in a pronounced stimulation of cholesterol 7 alpha-hydroxylase activity and bile acid mass production, but decreased the perivenous/periportal ratio of both parameters. These results demonstrate that bile acid mass production, but decreased the perivenous hepatocytes, possibly owing to feedback suppression by bile acids from the enterohepatic circulation. Furthermore, the opposite acinar localization of cholesterol and bile acid biosynthesis provides an interesting alternative to current views of the regulation of their metabolic pathways. Subject Health To reference this document use: http://resolver.tudelft.nl/uuid:8585bbac-c57d-4282-bd0d-2da47b0e88a8 TNO identifier 244164 Source Biochemical journal, 276 (276), 73-77 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.