Title
Heterogeneity of rat liver parenchyma in cholesterol 7α-hydroxylase and bile acids synthesis
Author
Gezondheidsorganisatie TNO Gaubius Instituut TNO
Ugele, B.
Kempen, J.M.
Gebhardt, R.
Mijer, P.
Burger, H.J.
Princen, H.M.G.
Publication year
1991
Abstract
Periportal and perivenous hepatocytes were isolated from rat liver by digitonin/collagenase perfusion for investigating the acinar distribution of bile acid synthesis. The specific activity of cholesterol 7 alpha-hydroxylase (EC 1.14.13.17) was 7.9-fold higher in perivenous cells than in periportal hepatocytes. Mass production of bile acids differed 4.4-fold between cultured perivenous and periportal hepatocytes. In contrast, the levels of free cholesterol in homogenates and microsomes derived from both subfractions were similar. Feeding of rats with the bile-acid-sequestering anion-exchange resin colestid resulted in a pronounced stimulation of cholesterol 7 alpha-hydroxylase activity and bile acid mass production, but decreased the perivenous/periportal ratio of both parameters. These results demonstrate that bile acid mass production, but decreased the perivenous hepatocytes, possibly owing to feedback suppression by bile acids from the enterohepatic circulation. Furthermore, the opposite acinar localization of cholesterol and bile acid biosynthesis provides an interesting alternative to current views of the regulation of their metabolic pathways.
Subject
Health
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TNO identifier
244164
Source
Biochemical journal, 276 (276), 73-77
Document type
article