Title
A convenient and reproducible method to genetically transform bacteria of the genus Bifidobacterium
Author
Argnani, A.
Leer, R.J.
van Luijk, N.
Pouwels, P.H.
TNO Voeding
Publication year
1996
Abstract
A protocol was developed for the introduction of foreign plasmid DNA into various Bifidobacterium strains. The method, which is applicable to all Bifidobacterium species tested so far, is based on electroporation of bacteria made competent by preincubation in electroporation buffer for several hours at 4°C. Transformation of Bifidobacterium could be achieved with a plasmid vector originating from Bifidobacterium and with plasmid vectors from Corynebacterium, but not with vectors carrying replicons from Lactococcus or Lactobacillus.
Subject
Nutrition
Electrotransformation
Plasmid vector
Plasmid dna
Bacterium competence
Vontrolled study
Expression vector
Genetic transformation
Lactobacillus bifidus
Methodology
Nonhuman
Priority journal
Amino Acid Sequence
Bifidobacterium
Corynebacterium
DNA Replication
Electroporation
Genes, Bacterial
Genetic Vectors
Lactobacillus
Lactococcus
Molecular Sequence Data
Plasmids
Reproducibility of Results
Sequence Homology, Amino Acid
Species Specificity
Transformation, Genetic
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TNO identifier
233155
ISSN
1350-0872
Source
Microbiology, 142 (1), 109-114
Document type
article