Print Email Facebook Twitter Elevation of cartilage AGEs does not accelerate initiation of canine experimental osteoarthritis upon mild surgical damage Title Elevation of cartilage AGEs does not accelerate initiation of canine experimental osteoarthritis upon mild surgical damage Author Vos, P.A.J.M. Degroot, J. Barten-Van Rijbroek, A.D. Zuurmond, A.-M. Bijlsma, J.W.J. Mastbergen, S.C. Lafeber, F.P.J.G. Publication year 2012 Abstract Osteoarthritis is a highly prevalent disease, age being the main risk factor. The age-related accumulation of advanced-glycation-endproducts (AGEs) adversely affects the mechanical and biochemical properties of cartilage. The hypothesis that accumulation of cartilage AGEs in combination with surgically induced damage predisposes to the development of osteoarthritis was tested in vivo in a canine model. To artificially increase cartilage AGEs, right knee joints of eight dogs were repeatedly injected with ribose/threose (AGEd-joints). Left joints with vehicle alone served as control. Subsequently, minimal surgically applied cartilage damage was induced and loading restrained as much as possible. Thirty weeks after surgery, joint tissues of all dogs were analyzed for biochemical and histological features of OA. Cartilage pentosidine levels were ∼5-fold enhanced (p = 0.001 vs. control-joints). On average, no statistically significant differences in joint degeneration were found between AGEd and control-joints. Enhanced cartilage pentosidine levels did correlate with less cartilage proteoglycan release (R = -0.762 and R = -0.810 for total and newly-formed proteoglycans, respectively; p = 0.028 and 0.015 for both). The current data support the diminished cartilage turnover, but only a tendency towards enhanced cartilage damage in AGEd articular cartilage was observed. As such, elevated AGEs do not unambiguously accelerate the development of early canine OA upon minimal surgical damage. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. Chemicals/CAS: pentosidine, 124505-87-9; ribose, 34466-20-1, 50-69-1, 93781-19-2 Subject LifeMHR - Metabolic Health Research PHS - Pharmacokinetics & Human StudiesEELS - Earth, Environmental and Life SciencesHealthy for LifeHealthHealthy Livingcaninenon-enzymatic glycationosteoarthritispentosidineproteoglycanadvanced glycation end productcarbohydratepentosidineproteoglycanribosethreoseunclassified druganimal cellanimal experimentanimal modelanimal tissuearticlebone turnovercartilagecartilage cellcartilage injurycontrolled studyexperimental dogfemalehistopathologyin vivo studyjoint degenerationknee surgerynonhumanosteoarthritispriority journalsynovitistissue level To reference this document use: http://resolver.tudelft.nl/uuid:84d114e3-0d72-4f68-9a4c-2459b24d0b6d DOI https://doi.org/10.1002/jor.22092 TNO identifier 462851 ISSN 0736-0266 Source Journal of Orthopaedic Research, 30 (9), 1398-1404 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.