Title
Elevation of cartilage AGEs does not accelerate initiation of canine experimental osteoarthritis upon mild surgical damage
Author
Vos, P.A.J.M.
Degroot, J.
Barten-Van Rijbroek, A.D.
Zuurmond, A.-M.
Bijlsma, J.W.J.
Mastbergen, S.C.
Lafeber, F.P.J.G.
Publication year
2012
Abstract
Osteoarthritis is a highly prevalent disease, age being the main risk factor. The age-related accumulation of advanced-glycation-endproducts (AGEs) adversely affects the mechanical and biochemical properties of cartilage. The hypothesis that accumulation of cartilage AGEs in combination with surgically induced damage predisposes to the development of osteoarthritis was tested in vivo in a canine model. To artificially increase cartilage AGEs, right knee joints of eight dogs were repeatedly injected with ribose/threose (AGEd-joints). Left joints with vehicle alone served as control. Subsequently, minimal surgically applied cartilage damage was induced and loading restrained as much as possible. Thirty weeks after surgery, joint tissues of all dogs were analyzed for biochemical and histological features of OA. Cartilage pentosidine levels were ∼5-fold enhanced (p = 0.001 vs. control-joints). On average, no statistically significant differences in joint degeneration were found between AGEd and control-joints. Enhanced cartilage pentosidine levels did correlate with less cartilage proteoglycan release (R = -0.762 and R = -0.810 for total and newly-formed proteoglycans, respectively; p = 0.028 and 0.015 for both). The current data support the diminished cartilage turnover, but only a tendency towards enhanced cartilage damage in AGEd articular cartilage was observed. As such, elevated AGEs do not unambiguously accelerate the development of early canine OA upon minimal surgical damage. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. Chemicals/CAS: pentosidine, 124505-87-9; ribose, 34466-20-1, 50-69-1, 93781-19-2
Subject
Life
MHR - Metabolic Health Research PHS - Pharmacokinetics & Human Studies
EELS - Earth, Environmental and Life Sciences
Healthy for Life
Health
Healthy Living
canine
non-enzymatic glycation
osteoarthritis
pentosidine
proteoglycan
advanced glycation end product
carbohydrate
pentosidine
proteoglycan
ribose
threose
unclassified drug
animal cell
animal experiment
animal model
animal tissue
article
bone turnover
cartilage
cartilage cell
cartilage injury
controlled study
experimental dog
female
histopathology
in vivo study
joint degeneration
knee surgery
nonhuman
osteoarthritis
priority journal
synovitis
tissue level
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http://resolver.tudelft.nl/uuid:84d114e3-0d72-4f68-9a4c-2459b24d0b6d
DOI
https://doi.org/10.1002/jor.22092
TNO identifier
462851
ISSN
0736-0266
Source
Journal of Orthopaedic Research, 30 (9), 1398-1404
Document type
article