Title
Further studies on mobilization of CFUs
Author
Vos, O.
Wilschut, I.J.C.
Medisch Biologisch Laboratorium TNO
Publication year
1979
Abstract
Mobilization of CFUs from haemopoietic tissues into circulation was studied after injection of different bacterial lipopolysaccharides (LPS), zymosan, phytohaemagglutinin (PHA), concanavalin A (Con A), trypsin and di-isopropyl-fluorophosphate-inhibited trypsin. All bacterial LPS used gave an increase of CFUs in the peripheral blood at 1 h after i.v. injection. Some variation in activity could not be excluded. As with Salmonella typhosa LPS, zymosan gave an increase in circulating CFUs during the first few hr and a second peak a few days later. After injection of zymosan as well as S. typhosa LPS the second peak in the blood was accompanied by a large increase in CFUs numbers in the spleen. PHA gave an immediate mobilization of CFUs, but the mobilization after injection of Con A during the first few hr occurred more slowly. After injection of S. typhosa LPS, zymosan and PHA the blood C3 level was found to be depressed considerably. This might indicate that the complement system is involved in the early mobilization of CFUs. Dexamethasone, a synthetic hormone which has been reported to give sequestration of several cell types in the bone marrow, did not inhibit the early and late mobilization of CFUs which normally occurs after injection of S. typhosa LPS. Copyright © 1979, Wiley Blackwell. All rights reserved
Subject
Biology
Animal experiment
Blood and hemopoietic system
Bone marrow
Colony forming unit
Hematopoietic cell
Mouse
Animal
Clonogenic assay
Comparative study
Complement activation
Cytology
Drug effect
Hematopoietic stem cell
Male
Physiology
Salmonella typhi
Colony-Forming Units Assay
Complement 3
Complement Activation
Concanavalin A
Dexamethasone
Hematopoietic Stem Cells
Mice
Phytohemagglutinins
Salmonella typhi
Complement component C3, 80295-41-6
Concanavalin A, 11028-71-0
Dexamethasone, 50-02-2
Dyflos, 55-91-4;
Phytohemagglutinin, 9008-97-3
Trypsin EC 3.4.21.4, 9002-07-7
Zymosan, 58856-93-2, 68652-43-7, 9010-72-4
Complement 3
Concanavalin A, 11028-71-0
Isoflurophate, 55-91-4
Lipopolysaccharides
To reference this document use:
http://resolver.tudelft.nl/uuid:81b36311-3bf6-428c-b0b2-b767a235be80
DOI
https://doi.org/10.1111/j.1365-2184.1979.tb00148.x
TNO identifier
572708
ISSN
0960-7722
Source
Cell Tissue Kinetics, 12 (3), 257-267
Document type
article