Print Email Facebook Twitter Wet-wrap treatment using dilutions of tacrolimus ointment and fluticasone propionate cream in human APOC1 (+/+) mice with atopic dermatitis Title Wet-wrap treatment using dilutions of tacrolimus ointment and fluticasone propionate cream in human APOC1 (+/+) mice with atopic dermatitis Author Oranje, A.P. Verbeek, R. Verzaal, P. Haspels, I. Prens, E. Nagelkerken, L. TNO Kwaliteit van Leven Publication year 2009 Abstract Background: Wet-wrap treatment (WWT) with diluted topical steroids is widely used in atopic dermatitis (AD). Mice with transgenic overexpression of human apolipoprotein C1 (APOC1) in the liver and the skin are not only characterized by hyperlipidaemia and raised IgE levels, but also by pruritic dermatitis and a disturbed skin barrier function, providing a novel in vivo mouse model for AD. Objectives: We investigated an adapted WWT method in the AD model in APOC1 mice in order to establish its efficacy. Methods: The effect of topical 0.1% and 0.03% tacrolimus ointment, tacrolimus base ointment, different dilutions of 0.05% fluticasone propionate (FP) cream and emollient on the development of dermatitis in APOC1 mice was investigated. WWT was performed with 0.03% tacrolimus ointment or 0.017% FP cream. Results: AD in APOC1 mice responded to topical treatment with tacrolimus or FP. In contrast to tacrolimus treatment, FP treatment was associated with loss of body weight. WWT reinforced several therapeutic aspects, notably improvements in transepidermal water loss and in epidermal thickness. WWT using tacrolimus 0.03% ointment was more effective than WWT using FP 0.017% cream. Conclusions: AD in APOC1 mice responds to treatment with (diluted) tacrolimus or FP; treatment with FP cream, but not tacrolimus ointment, was associated with weight loss. In this study, the adapted WWT using tacrolimus or FP in mice had a limited improving effect as compared with open application of tacrolimus or FP. © 2008 The Authors. Subject BiologyBiomedical ResearchApolipoprotein CAtopic dermatitisMouse modelTransepidermal water lossWet-wrap treatmentapolipoprotein C1fluticasone propionatetacrolimusanimal experimentanimal modelanimal tissuearticleatopic dermatitisclinical featurecontrolled studydisease severitydrug effectdrug formulationhistopathologymousenonhumanocclusive dressingpriority journalskin water lossskinfold thicknesstreatment responseweight reductionAndrostadienesAnimalsApolipoprotein C-IBandagesDermatitis, AtopicDisease ProgressionDose-Response Relationship, DrugEmollientsHumansMiceMice, TransgenicModels, AnimalOintmentsTacrolimus To reference this document use: http://resolver.tudelft.nl/uuid:81a2a3d9-c4bc-4c93-893f-5c2cb8fd2c20 DOI https://doi.org/10.1111/j.1365-2133.2008.08834.x TNO identifier 241338 ISSN 0007-0963 Source British Journal of Dermatology, 160 (1), 54-61 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.