Title
Retrospective detection of exposure to organophosphorus anti-cholinesterases: Mass spectrometric analysis of phosphylated human butyrylcholinesterase
Author
Fidder, A.
Hulst, A.G.
Noort, D.
de Ruiter, R.
van der Schans, M.J.
Benschop, H.P.
Langenberg, J.P.
Prins Maurits Laboratorium TNO
Publication year
2002
Abstract
In this paper a novel and general procedure is presented for detection of organophosphate-inhibited human butyrylcholinesterase (HuBuChE), which is based on electrospray tandem mass spectrometric analysis of phosphylated nonapeptides obtained after pepsin digestion of the enzyme. The utility of this method is exemplified by the positive analysis of serum samples from Japanese victims of the terrorist attack with sarin in the Tokyo subway in 1995.
Subject
Warfare
Cholinesterase
Cholinesterase inhibitor
Nerve gas
Organophosphorus compound
Pronase
Sarin
Article
Colorimetry
Enzyme activity
Enzyme assay
High performance liquid chromatography
Hydrolysis
Intoxication
Mass spectrometry
Protein phosphorylation
Spectrometry
Synthesis
Butyrylcholinesterase
Cholinesterase Inhibitors
Environmental Exposure
Environmental Monitoring
Humans
Insecticides
Peptide Fragments
Sarin
Spectrometry, Mass, Electrospray Ionization
Terrorism
Butyrylcholinesterase, EC 3.1.1.-
Cholinesterase Inhibitors
Insecticides
Peptide fragments
Sarin, 107-44-8
To reference this document use:
http://resolver.tudelft.nl/uuid:7db2708a-2e28-43e4-aad0-bac2b4241d1c
DOI
https://doi.org/10.1021/tx0101806
TNO identifier
236527
ISSN
0893-228X
Source
Chemical Research in Toxicology, 15 (4), 582-590
Document type
article