Print Email Facebook Twitter Improvements in the 32P-postlabelling procedure to quantify bulky aromatic DNA adducts Title Improvements in the 32P-postlabelling procedure to quantify bulky aromatic DNA adducts Author Steenwinkel, M.J.S.T. Roggeband, R. van Delft, J.H.M. Baan, R.A. Contributor Phillips, D.H. (editor) Castegnaro, M. (editor) Bartsch, H. (editor) Publication year 1993 Abstract This contribution describes methodological modifications and improvements that may contribute to inter-assay reproducibility and more accurate adduct quantification for 32P-postlabelling. Firstly, an anion-exchange chromatography procedure was developed to determine the amount of DNA used per assay and to check its purity, in particular to verify the absence of contaminating RNA. Secondly, calibration standards were prepared, in order to correct for differences in recovery. The modification levels of these standards were determined by synchronous fluorescence spectrophotometric analysis. Thirdly, the effect on adduct levels of exposure to light during postlabelling was investigated. Exposure of polyaromatic DNA adducts on a PEI-cellulose plate reduced the amounts of adducts detected considerably. Chemicals/CAS: DNA, 9007-49-2; phosphorus, 7723-14-0; RNA, 63231-63-0; Carcinogens; DNA, 9007-49-2; Phosphorus Radioisotopes; Polycyclic Hydrocarbons; RNA, 63231-63-0 Subject PhosphorusPolycyclic hydrocarbonIon exchange chromatographyIsolation and purificationLightMethodologyReproducibilityStandardCarcinogensChromatography, Ion ExchangeDNADNA DamageEnvironmental MonitoringEvaluation StudiesHumanLightOccupational ExposurePhosphorus RadioisotopesPolycyclic HydrocarbonsReference StandardsReproducibility of ResultsRNA To reference this document use: http://resolver.tudelft.nl/uuid:7a065a2f-73f7-4ae3-8a19-e18b0e8966ea TNO identifier 82307 Publisher IARC, Lyon Source Postlabelling methods for detection of DNA adducts, 65-70 Series IARC Scientific Publications Bibliographical note Human and Experimental Toxicology, (12)4, 351 Document type bookPart Files To receive the publication files, please send an e-mail request to TNO Library.