Title
Invasive properties of fibroblast-like synoviocytes Correlation with growth characteristics and expression of MMP-1 MMP-3 and MMP-10
Author
TNO Preventie en Gezondheid
Tolboom, T.C.A.
Pieterman, E.
van der Laan, W.H.
Toes, R.E.M.
Huidekoper, A.L.
Nelissen, R.G.H.H.
Breedveld, F.C.
Huizinga, T.W.J.
Publication year
2002
Abstract
Background: Matrix metalloproteinases (MMPs) have a pivotal role in the destruction of cartilage in rheumatoid arthritis (RA), which is mediated by the fibroblast-like synoviocytes (FLS). Objective: To examine the in vitro invasiveness of synoviocytes obtained from inflamed joints of patients with arthritis in relation to the expression of MMP 1-14, 17, 19, cathepsin-K, the tissue inhibitors of matrix metalloproteinases TIMP-1 and TIMP-2 by FLS. Methods: FLS were derived from 56 patients (30 with RA, 17 with osteoarthritis (OA), and nine with avascular necrosis (AVN)). Invasive growth of FLS through an artificial matrix (Matrigel) was measured in a transwell system. The number of cells that migrated through the matrix were counted. Proliferation rate was determined by counting the FLS after seven days of culturing. Expression of MMPs, cathepsin-K and TIMPs was investigated with reverse transcriptase-polymerase chain reaction and related to the expression of a household gene, ?-actin. Results: FLS from RA showed greater invasive growth than FLS from OA and AVN. The median number of cells that grew through the matrix membrane was 4788 for RA, significantly higher than the number for OA, 1875 (p
Subject
beta actin
cathepsin K
collagenase 3
gelatinase A
gelatinase B
interstitial collagenase
macrophage elastase
matrigel
matrilysin
matrix metalloproteinase
matrix metalloproteinase 14
matrix metalloproteinase 17
matrix metalloproteinase 19
messenger RNA
neutrophil collagenase
stromelysin
stromelysin 2
stromelysin 3
tissue inhibitor of metalloproteinase 1
tissue inhibitor of metalloproteinase 2
unclassified drug
adult
aged
article
avascular necrosis
cartilage degeneration
cell growth
cell invasion
cell proliferation
correlation analysis
enzyme linked immunosorbent assay
extracellular matrix
fibroblast
gene expression
human
human tissue
major clinical study
osteoarthritis
priority journal
protein determination
protein expression
reverse transcription polymerase chain reaction
rheumatoid arthritis
RNA analysis
synoviocyte
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DOI
https://doi.org/10.1136/ard.61.11.975
TNO identifier
953628
ISSN
0003-4967
Source
Annals of the Rheumatic Diseases, 61 (61), 975-980
Document type
article