Title
Predicted serum folate concentrations based on in vitro studies and kinetic modeling are consistent with measured folate concentrations in humans
Author
Verwei, M.
Freidig, A.P.
Havenaar, R.
Groten, J.P.
TNO Kwaliteit van Leven
Publication year
2006
Abstract
The nutritional quality of new functional or fortified food products depends on the bioavailability of the nutrient(s) in the human body. Bioavailability is often determined in human intervention studies by measurements of plasma or serum profiles over a certain time period. These studies are time and cost consuming and often appear to lack an optimal study design, leading to follow-up intervention trials. Therefore, an alternative approach is needed that will optimize the development of new products. This study describes an approach to predict human serum concentrations after the consumption of (fortified) food products. The concept is based on the integration of in vitro results with kinetic modeling. As a case study, human serum folate concentrations were predicted after the consumption of folate-fortified milk products for 4 wk. Oral bioavailability was investigated using a step-wise approach in which luminal bioaccessibility and intestinal absorption were independently evaluated. Subsequently, these in vitro data were integrated in a kinetic mathematical (in silico) model to predict serum folate concentrations after the intake of a single dose and during long-term consumption. This approach was evaluated in comparison to a human intervention study in which folic acid-fortified milk products were tested for their effect on serum folate concentrations. A high predictive quality of this alternative in vitro/in silico approach was demonstrated. Finally, this methodology was applied to predict serum folate concentrations after intake of different fortified milk products for 4 wk, showing its benefits for the development of new nutritional products. © 2006 American Society for Nutrition.
Subject
Biology
Biomedical Research
endogenous compound
folic acid
article
clinical trial
computer model
controlled clinical trial
controlled study
folic acid blood level
follow up
food industry
human
in vitro study
kinetics
milk
nutritional status
Dairy Products
Folic Acid
Humans
Ileum
Intestinal Absorption
Jejunum
Kinetics
Models, Biological
Reproducibility of Results
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http://resolver.tudelft.nl/uuid:7925187e-c634-4569-9e63-1b8c50ad8193
TNO identifier
239631
ISSN
0022-3166
Source
Journal of Nutrition, 136 (12), 3074-3078
Document type
article