Print Email Facebook Twitter Human granzyme B mediates cartilage proteoglycan degradation and is expressed at the invasive front of the synovium in rheumatoid arthritis Title Human granzyme B mediates cartilage proteoglycan degradation and is expressed at the invasive front of the synovium in rheumatoid arthritis Author Ronday, H.K. van der Laan, W.H. Tak, P.P. de Roos, J.A.D.M. Bank, R.A. Tekoppele, J.M. Froelich, C.J. Hack, C.E. Hogendoorn, P.C.W. Breedveld, F.C. Verheijen, J.H. Gaubius Instituut Publication year 2001 Abstract Objective. To investigate the cartilage-degrading capacity of granzyme B and the presence of granzyme B-positive cells at sites of erosion in the rheumatoid synovium. Methods. Granzyme B was added to [3H]proline/[35S]sulphate-labelled cartilage matrices and to cartilage explants. Proteoglycan degradation was assessed by the release of 35S and glycosaminoglycans into the medium and collagen degradation was assessed by the release of 3H and hydroxyproline and by measuring the fraction of denatured collagen. Granzyme B expression was studied at the invasive front of the synovium by immunohistochemistry. Results. Granzyme B induced loss of both newly synthesized, radiolabelled proteoglycans in cartilage matrices and resident proteoglycans of the cartilage explants. No effect on collagen degradation was found. Granzyme B-positive cells were present throughout the synovium and at the invasive front. Conclusion. The presence of granzyme B-positive cells at the invasive front of the synovium together with its ability to degrade articular proteoglycans supports the view that granzyme B may contribute to joint destruction in rheumatoid arthritis. Subject Biology HealthBiomedical ResearchCartilage destructionGranzyme BRheumatoid arthritiscollagenglycosaminoglycangranzyme Bhydroxyprolineprolineproteoglycansulfatesulfur 35tritiumanimal tissuebone erosioncartilage degenerationcartilage matrixcollagen degradationcontrolled studyexplanthuman cellimmunohistochemistryisotope labelingjoint destructionnonhumanpriority journalprotein denaturationprotein expressionproteoglycan synthesisrheumatoid arthritissynoviumAnimalsArthritis, RheumatoidCartilageCattleCells, CulturedChondrocytesCollagenExtracellular MatrixGranzymesHumansMetacarpophalangeal JointProteoglycansSerine EndopeptidasesSynovial Membrane To reference this document use: http://resolver.tudelft.nl/uuid:76072712-13cf-479d-9980-495ce1c16d5f TNO identifier 235982 ISSN 1462-0324 Source Rheumatology, 40 (1), 55-61 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.