Print Email Facebook Twitter The metabolism in vitro of human low-density lipoprotein by the human hepatoma cell line Hep G2 Title The metabolism in vitro of human low-density lipoprotein by the human hepatoma cell line Hep G2 Author Gaubius instituut TNO Havekes, L. van Hinsbergh, V. Kempen, H.J. Emeis, J. Publication year 1983 Abstract The human hepatoma cell line Hep G2 was studied with respect to metabolism of human low-density lipoprotein (LDL). The Hep G2 cells bind, take up and degrade human LDL with a high-affinity saturable and with a low-affinity non-saturable component. The high-affinity binding possesses a K(D) of 25 nM-LDL and a maximal amount of binding of about 70 ng of LDL-apoprotein/mg of cell protein. The high-affinity binding, uptake and degradation of LDL by Hep G2 cells is dependent on the extracellular Ca2+ concentration and is down-regulated by the presence of fairly high concentrations of extracellular LDL. Incubation of the Hep G2 cells with LDL results in suppression of the intracellular cholesterol synthesis. It is concluded that the human hepatoma cell line hep G2 possesses specific LDL receptors similar to the LDL receptors demonstrated on extrahepatic tissue cells. Chemicals/CAS: Blood Proteins; Cholesterol, 57-88-5; Edetic Acid, 60-00-4; Lipoproteins, LDL; Receptors, Cell Surface; Receptors, LDL Subject low density lipoproteinhepatoma cellhumanin vitro studyliverBlood ProteinsCarcinoma, HepatocellularCell LineCholesterolEdetic AcidHumanKineticsLipoproteins, LDLLiver NeoplasmsMicroscopy, FluorescenceProtein BindingReceptors, Cell SurfaceReceptors, LDL To reference this document use: http://resolver.tudelft.nl/uuid:75e6a0c1-6fb8-4efb-83ef-3d99eec0a623 TNO identifier 229362 ISSN 0264-6021 Source Biochemical Journal, 214 (214), 951-958 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.