Title
The metabolism in vitro of human low-density lipoprotein by the human hepatoma cell line Hep G2
Author
Gaubius instituut TNO
Havekes, L.
van Hinsbergh, V.
Kempen, H.J.
Emeis, J.
Publication year
1983
Abstract
The human hepatoma cell line Hep G2 was studied with respect to metabolism of human low-density lipoprotein (LDL). The Hep G2 cells bind, take up and degrade human LDL with a high-affinity saturable and with a low-affinity non-saturable component. The high-affinity binding possesses a K(D) of 25 nM-LDL and a maximal amount of binding of about 70 ng of LDL-apoprotein/mg of cell protein. The high-affinity binding, uptake and degradation of LDL by Hep G2 cells is dependent on the extracellular Ca2+ concentration and is down-regulated by the presence of fairly high concentrations of extracellular LDL. Incubation of the Hep G2 cells with LDL results in suppression of the intracellular cholesterol synthesis. It is concluded that the human hepatoma cell line hep G2 possesses specific LDL receptors similar to the LDL receptors demonstrated on extrahepatic tissue cells. Chemicals/CAS: Blood Proteins; Cholesterol, 57-88-5; Edetic Acid, 60-00-4; Lipoproteins, LDL; Receptors, Cell Surface; Receptors, LDL
Subject
low density lipoprotein
hepatoma cell
human
in vitro study
liver
Blood Proteins
Carcinoma, Hepatocellular
Cell Line
Cholesterol
Edetic Acid
Human
Kinetics
Lipoproteins, LDL
Liver Neoplasms
Microscopy, Fluorescence
Protein Binding
Receptors, Cell Surface
Receptors, LDL
To reference this document use:
http://resolver.tudelft.nl/uuid:75e6a0c1-6fb8-4efb-83ef-3d99eec0a623
TNO identifier
229362
ISSN
0264-6021
Source
Biochemical Journal, 214 (214), 951-958
Document type
article