Print Email Facebook Twitter Phylogeny of fungal hemoglobins and expression analysis of the Aspergillus oryzae flavohemoglobin gene fhbA during hyphal growth Title Phylogeny of fungal hemoglobins and expression analysis of the Aspergillus oryzae flavohemoglobin gene fhbA during hyphal growth Author te Biesebeke, R. Levasseur, A. Boussier, A. Record, E. van den Hondel, C.A.M.J.J. Punt, P.J. TNO Kwaliteit van Leven Publication year 2010 Abstract The fhbA genes encoding putative flavohemoglobins (FHb) from Aspergillus niger and Aspergillus oryzae were isolated. Comparison of the deduced amino acid sequence of the A. niger fhbA gene and other putative filamentous fungal FHb-encoding genes to that of Ralstonia eutropha shows an overall conserved gene structure and completely conserved catalytic amino acids. Several yeasts and filamentous fungi, including both Aspergillus species have been found to contain a small FHb gene family mostly consisting of two family members. Based on these sequences the evolutionary history of the fungal FHb family was reconstructed. The isolated fhbA genes from A. oryzae and A. niger belong to a phylogenetic group, which exclusively contains Aspergillus genes. Different experimental approaches show that fhbA transcript levels appear during active hyphal growth. Moreover, in a pclA-disrupted strain with a hyperbranching growth phenotype, the transcript levels of the fhbA gene were 2-5 times higher compared to the wild-type. These results suggest that FHb from filamentous fungi have a function that is correlated to the hyphal growth phenotype. © 2009. Subject BiologyAspergillus oryzaefhbA transcriptionFlavohemoglobinPhylogenyPolarized fungal growth To reference this document use: http://resolver.tudelft.nl/uuid:74edbcfe-a1c5-420c-a459-8ac9db3f4848 DOI https://doi.org/10.1016/j.mycres.2009.08.007 TNO identifier 346426 ISSN 1878-6146 Source Fungal Biology, 114 (2-3), 135-143 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.