Print Email Facebook Twitter High-protein and high-carbohydrate breakfasts differentially change the transcriptome of human blood cells Title High-protein and high-carbohydrate breakfasts differentially change the transcriptome of human blood cells Author van Erk, M.J. Blom, W.A.M. van Ommen, B. Hendriks, H.F.J. TNO Kwaliteit van Leven Publication year 2006 Abstract Background: Application of transcriptomics technology in human nutrition intervention studies would allow for genome-wide screening of the effects of specific diets or nutrients and result in biomarker profiles. Objective: The aim was to evaluate the potential of gene expression profiling in blood cells collected in a human intervention study that investigated the effect of a high-carbohydrate (HC) or a high-protein (HP) breakfast on satiety. Design: Blood samples were taken from 8 healthy men before and 2 h after consumption of an HP or an HC breakfast. Both breakfasts contained acetaminophen for measuring the gastric emptying rate. Analysis of the transcriptome data focused on the effects of the HP or HC breakfast and of acetaminophen on blood leukocyte gene expression profiles. Results: Breakfast consumption resulted in differentially expressed genes, 317 for the HC breakfast and 919 for the HP breakfast. Immune response and signal transduction, specifically T cell receptor signaling and nuclear transcription factor κB signaling, were the overrepresented functional groups in the set of 141 genes that were differentially expressed in response to both breakfasts. Consumption of the HC breakfast resulted in differential expression of glycogen metabolism genes, and consumption of the HP breakfast resulted in differential expression of genes involved in protein biosynthesis. Conclusions: Gene expression changes in blood leukocytes corresponded with and may be related to the difference in macronutrient content of the breakfast, meal consumption as such, and acetaminophen exposure. This study illustrates the potential of gene expression profiling in blood to study the effects of dietary exposure in human intervention studies. © 2006 American Society for Nutrition. Subject NutritionBiomedical ResearchAcetaminophenCarbohydrateGene expressionHuman bloodLeukocytesProteinimmunoglobulin enhancer binding proteinparacetamolT lymphocyte receptoradultarticleblood cellblood samplingcarbohydrate dietfood intakegene expression profilinggene overexpressionglycogen metabolismhumanhuman experimentimmune responseleukocytemalenormal humanprotein dietprotein synthesissignal transductionstomach emptyingAcetaminophenAdolescentAdultCross-Over StudiesDietary CarbohydratesDietary ProteinsEatingGastric EmptyingGene ExpressionGene Expression ProfilingGene Expression RegulationHumansLeukocytesMaleNF-kappa BOligonucleotide Array Sequence AnalysisReceptors, Antigen, T-CellRNASatiationSignal TransductionSingle-Blind Method To reference this document use: http://resolver.tudelft.nl/uuid:729599c7-adf7-4a94-a230-87b759ebcb0e TNO identifier 239552 ISSN 0002-9165 Source American Journal of Clinical Nutrition, 84 (5), 1233-1241 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.