Title
Recombinant Lactobacillus plantarum inhibits house dust mite-specific T-cell responses
Author
Kruisselbrink, A.
Heijne Den Bak-Glashouwer, M.J.
Havenith, C.E.G.
Thole, J.E.R.
Janssen, R.
Publication year
2001
Abstract
Recent evidence suggests that chronic exposure to lactobacilli, which are part of the normal intestinal flora, inhibits the development of allergic disorders. Allergy is mediated by Th2 cells, which produce high levels of IL4 and IL5, and suppressive effects of lactic acid bacteria on the development of allergy have been attributed to their Th1-inducing properties. On the other hand, lactic acid bacteria have also been shown to suppress autoimmune disorders which are mediated by Th1 cells producing high levels of IFNγ. To study this apparent discrepancy, the immunomodulatory potential of lactobacilli was evaluated using recombinants that express an immunodominant T-cell epitope of Der p 1 of house dust mites. Mucosal immunization of C57BL/6 J mice with such recombinants resulted in the induction of T cells which produced low amounts of IFNγ. Immunization with the house dust mite peptide followed by treatment with recombinant Lactobacillus plantarum resulted in the inhibition of both IFNγ and IL5 production. The effect on IFNγ production was shown to be a non-specific effect of L. plantarum. The effect on IL5 production, however, was only observed when the recombinant expressing the Der p 1 peptide, but not the control recombinant, was used for treatment. Neither of the recombinants had an effect on the antibody response. Taken together, these data suggest that recombinant L. plantarum may be a suitable candidate for the treatment of allergic disorders. Chemicals/CAS: Antigens, Dermatophagoides; Dust; Glycoproteins; Immunoglobulin E, 37341-29-0; Immunoglobulin G; Interferon Type II, 82115-62-6; Interleukin-5; Recombinant Fusion Proteins
Subject
Health
Allergy
Mucosal immunity
Th1/Th2
Antibody
Epitope
Gamma interferon
Peptide
Peptide der p 1
Unclassified drug
Allergic disease
Animal cell AAnimal experiment
Animal model
Animal tissue
Antibody response
Controlled study
Cytokine production
Dermatophagoides pteronyssinus
Immune response
Immunization
Immunomodulation
Lactobacillus plantarum
Mouse
Nonhuman
T lymphocyte
Animals
Antigens, Dermatophagoides
Cells, Cultured
Dust
Female
Glycoproteins
Hypersensitivity, Immediate
Immunoglobulin E
Immunoglobulin G
Interferon Type II
Interleukin-5
Lactobacillus
Lymphocyte Activation
Mice
Mice, Inbred C57BL
Mites
Recombinant Fusion Proteins
T-Lymphocytes
Th1 Cells
Th2 Cells
Transformation, Bacterial
To reference this document use:
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DOI
https://doi.org/10.1046/j.1365-2249.2001.01642.x
TNO identifier
236269
ISSN
0009-9104
Source
Clinical and Experimental Immunology, 126 (1), 2-8
Document type
article