Title
Relaxin stimulates MMP-2 and α-smooth muscle actin expression by human periodontal ligament cells
Author
Henneman, S.
Bildt, M.M.
de Groot, J.
Kuijpers-Jagtman, A.M.
Von den Hoff, J.W.
Publication year
2008
Abstract
The main cells in the periodontal ligament (PDL) are the fibroblasts, which play an important role in periodontal remodelling. Matrix metalloproteinases (MMPs) are largely responsible for the degradation of extracellular matrix proteins in the PDL. Previous studies have indicated that MMP production can be stimulated by the hormone relaxin. This hormone facilitates delivery by softening the connective tissues of the reproductive tract, and it prepares the mammary gland for lactation. Periodontal remodelling takes place during orthodontic tooth movement, which might be enhanced by relaxin. Therefore, we investigated the effects of relaxin on gelatinase expression of human PDL cells. Cultures of human PDL cells were incubated with relaxin. Gelatinase (MMP-2 and -9) expression, α-smooth muscle actin expression (α-SMA), total MMP activity and DNA content were measured. Both proMMP-2 and active MMP-2 was identified in the cultures. There was a clear trend showing a dose-dependent increase of MMP-2 production, which was significant at 250 ng/ml. Total MMP activity was not affected. A stimulation of α-SMA expression was found at 50 ng/ml. The results indicate that relaxin activates human PDL cells by the stimulation of MMP-2 and α-smooth muscle actin. © 2007 Elsevier Ltd. All rights reserved.
Subject
Biology Nutrition
α-SMA
Fibroblasts
MMP
Periodontal ligament
Relaxin
Gelatinase
Matrix metalloproteinase
Relaxin
Tissue inhibitor of metalloproteinase
Article
Cell culture
Chemistry
Cytology
Drug effect
Enzymology
Female
Fibroblast
Gene expression
Genetics
Human
Male
Metabolism
Periodontal ligament
Cells, Cultured
Female
Fibroblasts
Gelatinases
Gene Expression
Humans
Male
Matrix Metalloproteinases
Periodontal Ligament
Relaxin
Tissue Inhibitor of Metalloproteinases
To reference this document use:
http://resolver.tudelft.nl/uuid:71abceb2-5808-437b-960f-dad4eebb68e4
DOI
https://doi.org/10.1016/j.archoralbio.2007.08.010
TNO identifier
240652
ISSN
0003-9969
Source
Archives of Oral Biology, 53 (2), 161-167
Document type
article