Print Email Facebook Twitter Doxycycline inhibits collagen synthesis by bovine chondrocytes cultured in alginate Title Doxycycline inhibits collagen synthesis by bovine chondrocytes cultured in alginate Author Beekman, B. Verzijl, N. de Roos, J.A.D.M. Koopman, J.L. Tekoppele, J.M. Leiden, Netherlands Gaubius Instituut TNO Publication year 1997 Abstract Doxycycline is known for its ability to inhibit matrix metalloproteinases (MMPs), a family of enzymes that play a role in cartilage breakdown in arthritides. Its prophylactic effect in reducing joint degradation in osteoarthritis is mainly attributed to this property. In this study, we show that doxycycline exhibits a profound inhibition of collagen synthesis by bovine articular chondrocytes cultured in alginate. At 25 μM doxycycline, collagen synthesis was decreased by 50%; no effect on cell proliferation (DNA levels) or general protein synthesis (35S-Met and 35S-Cys incorporation) was observed. Messenger RNA levels of type II collagen were also reduced, indicating an effect of doxycycline at the transcriptional level. The concentration of doxycycline needed to downregulate collagen synthesis was > 10-fold lower than that needed to inhibit most of the MMPs. Inasmuch as differentiated chondrocytes in the early stages of osteoarthritis exhibit increased collagen synthesis, the beneficial effect of doxycycline in vivo may involve prevention of changes in chondrocyte phenotype. Chemicals/CAS: alginic acid, 28961-37-7, 29894-36-8, 9005-32-7, 9005-38-3; cysteine, 4371-52-2, 52-89-1, 52-90-4; doxycycline, 10592-13-9, 17086-28-1, 564-25-0; methionine, 59-51-8, 63-68-3, 7005-18-7; sulfur 35, 15117-53-0; Alginates; alginic acid, 9005-32-7; Collagen, 9007-34-5; Cysteine, 52-90-4; DNA, 9007-49-2; Doxycycline, 564-25-0; Glucuronic Acid, 576-37-4; Hexuronic Acids; Methionine, 63-68-3; Protein Synthesis Inhibitors; RNA, Messenger; Sulfur Radioisotopes Subject BiologyBiomedical Researchalginic acidcysteinemethioninecollagenglucuronic acidhexuronic acidmessenger RNAprotein synthesis inhibitorsulfurarticular cartilagecell culturecell proliferationcollagen synthesismetabolic inhibitionnonhumanosteoarthritisphenotypeanimalbiosynthesisculture techniquecytologydose responsedrug effectgenetic transcriptionmetabolismmetacarpophalangeal jointmethodologyprotein synthesisAlginatesAnimalsCartilage, ArticularCattleCell Culture TechniquesCell DivisionCell SurvivalCollagenCysteineDNADose-Response Relationship, DrugDoxycyclineGlucuronic AcidHexuronic AcidsKineticsMetacarpophalangeal JointMethionineProtein BiosynthesisProtein Synthesis InhibitorsRNA, MessengerSulfur RadioisotopesTranscription, Genetic To reference this document use: http://resolver.tudelft.nl/uuid:7114b32c-8c65-47ac-af9a-5f0a9020ce26 DOI https://doi.org/10.1006/bbrc.1997.7088 TNO identifier 234005 ISSN 0006-291X Source Biochemical and Biophysical Research Communications, 237 (1), 107-110 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.